LCK 抗体 (AA 1-191)
(5 references)Quick Overview for LCK 抗体 (AA 1-191) (ABIN967742)
抗原
See all LCK 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-191
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交叉反应
- 小鼠, 大鼠
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产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Human Lck aa. 1-191
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亚型
- IgG1
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anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-539) antibody
LCK 适用: 人 WB, ELISA, IP, RNAi 宿主: 小鼠 Monoclonal 3F7-F5 unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-240) antibodyLCK 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 22-36) antibodyLCK 适用: 人 WB, IP, ICC, ICFC 宿主: 小鼠 Monoclonal LCK-01 unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 23-52) antibodyLCK 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-539) antibodyLCK 适用: 人 ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 468-506), (C-Term) antibodyLCK 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 480-509) antibodyLCK 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 421-509) antibodyLCK 适用: 人, 小鼠 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 39-52) antibodyVerified LCK 适用: 人, 大鼠, Pig WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-539) antibodyLCK 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 250 μg/mL
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缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store undiluted at -20° C.
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: "LAT displacement from lipid rafts as a molecular mechanism for the inhibition of T cell signaling by polyunsaturated fatty acids." in: The Journal of biological chemistry, Vol. 277, Issue 32, pp. 28418-23, (2002) (PubMed).
: "Differential loss of T cell signaling molecules in metastatic melanoma patients' T lymphocyte subsets expressing distinct TCR variable regions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 12, pp. 6912-23, (2000) (PubMed).
: "Hierarchy of protein tyrosine kinases in interleukin-2 (IL-2) signaling: activation of syk depends on Jak3; however, neither Syk nor Lck is required for IL-2-mediated STAT activation." in: Molecular and cellular biology, Vol. 20, Issue 12, pp. 4371-80, (2000) (PubMed).
: "A kinase-independent function of Lck in potentiating antigen-specific T cell activation." in: Cell, Vol. 74, Issue 4, pp. 633-43, (1993) (PubMed).
: "T-lymphocyte interleukin 2-dependent tyrosine protein kinase signal transduction involves the activation of p56lck." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 5, pp. 1996-2000, (1991) (PubMed).
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: "LAT displacement from lipid rafts as a molecular mechanism for the inhibition of T cell signaling by polyunsaturated fatty acids." in: The Journal of biological chemistry, Vol. 277, Issue 32, pp. 28418-23, (2002) (PubMed).
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- LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))
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别名
- Lck
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背景
- The p56[lck] protein kinase is a member of the src family of cytoplasmic protein-tyrosine kinases (PTKs). Members of this family have several common features: 1) unique N-terminal domains, 2) attachment to cellular membranes through a myristylated N-terminus, and 3) homologous SH2, SH3, and catalytic domains. Within the src family of PTKs, lck, fyn, and Yes are expressed in T cells. The unique N-terminal domain of p56[lck] interacts with the cytoplasmic tails of the CD4 and CD8 cell surface glycoproteins. CD4 and CD8 bind to surface major histocompatibility complex (MHC) class II and class I molecules, respectively. These complexes interact with the T cell antigen receptor (TCR) in the early stages of T cell activation. In addition, an activated lck kinase increases responsiveness of some T cell hybridomas to antigen. The phosphorylation status and, therefore, the activity of p56[lck] kinase is regulated by the CD45 tyrosine protein phosphatase. Several studies suggest that lck has many functions critical to T cell development and activation. Mice lacking a functional lck gene are drastically impaired in the production of T lymphocytes. Variants of the human Jurkat T cell line that do not express p56[lck] exhibit a diminished response to stimulation of the T cell receptor. Evidence suggests that lck is directly upstream from PI3-kinase in the signal transduction cascade in T cell activation.
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分子量
- 56 kDa
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途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
抗原
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