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CDK1 抗体 (AA 283-297)

Name: CDK1 抗体 (AA 283-297)
SKU: ABIN967705
Availability: OutOfStock

This anti-CDK1 antibody is a 小鼠单克隆 antibody detecting CDK1 in WB, IF, IP 和 IHC (f). Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 5+ publications.

Western Blotting (WB) image for anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 283-297) antibody (ABIN967705) (CDK1 抗体 (AA 283-297))

Western blot analysis of Cdk1 on a HeLa cell lysate. (CDK1 抗体 (AA 283-297))

Immunoflourescence staining of human fibroblasts. (CDK1 抗体 (AA 283-297))

Image no. 4 for anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 283-297) antibody (ABIN967705) (CDK1 抗体 (AA 283-297))

4 images

(5 references)

产品编号 ABIN967705

发货至: 中国

Quick Overview for CDK1 抗体 (AA 283-297) (ABIN967705)

抗原

See all CDK1 抗体

CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

适用

人, 小鼠, 大鼠

宿主

小鼠

克隆类型

单克隆

标记

This CDK1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

1-Cdk1-Cdc2

Discover our top product CDK1 Primary Antibody

抗原表位
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21

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16

9

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4

4

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3

3

3

2

2

2

2

2

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2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 283-297

交叉反应

小鼠, 大鼠

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Human p34 [cdc2] aa. 283-297

亚型

IgG1
anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 59-182) antibody
CDK1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) (pTyr15) antibody
CDK1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 211-297) antibody
CDK1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1G10 unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 1-297) antibody
CDK1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A4-1A9 unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 208-258) antibody
CDK1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) antibody
CDK1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 211-297) antibody
CDK1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 8F1 unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 1-297) antibody
CDK1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 66-297) antibody
CDK1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 201-297) antibody
CDK1 适用: 人, 小鼠, 大鼠, 犬, 绵羊 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

说明

Related Products: ABIN968535, ABIN967389

限制

仅限研究用
状态

Liquid

浓度

250 μg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20° C.
García, Camacho, Morente, Fraga, Montalbán, Alvaro, Bellas, Castaño, Díez, Flores, Martin, Martinez, Mazorra, Menárguez, Mestre, Mollejo, Sáez, Sánchez, Piris: "Hodgkin and Reed-Sternberg cells harbor alterations in the major tumor suppressor pathways and cell-cycle checkpoints: analyses using tissue microarrays." in: Blood, Vol. 101, Issue 2, pp. 681-9, (2003) (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Takasaki, Kogure, Takeuchi, Kaneda, Yano, Hirokawa, Hirose, Shirai, Hashimoto: "Reactivity of anti-proliferating cell nuclear antigen (PCNA) murine monoclonal antibodies and human autoantibodies to the PCNA multiprotein complexes involved in cell proliferation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 7, pp. 4780-7, (2001) (PubMed).

Barboule, Lafon, Chadebech, Vidal, Valette: "Involvement of p21 in the PKC-induced regulation of the G2/M cell cycle transition." in: FEBS letters, Vol. 444, Issue 1, pp. 32-7, (1999) (PubMed).

Uckun, Tuel-Ahlgren, Waddick, Jun, Jin, Myers, Rowley, Burkhardt, Bolen: "Physical and functional interactions between Lyn and p34cdc2 kinases in irradiated human B-cell precursors." in: The Journal of biological chemistry, Vol. 271, Issue 11, pp. 6389-97, (1996) (PubMed).
抗原

CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

别名

Cdk1

背景

Cdk1, also known as p34 [cdc2], is a ubiquitously expressed serine/threonine protein kinase. Cdk1/cdc2 has been identified as the catalytic subunit of the maturation-promoting factor (MPF), while cyclin B acts as the regulatory subunit. The binding of these two subunits is critical to the transition into M-phase of the mammalian cell cycle, and this factor's role is regulated by a series of phosphorylations and dephosphorylations. After binding to cyclin B, cdk1/cdc2 is phosphorylated on Thr-14, by Myt1, and Tyr-15, by wee1 or mik1, yielding an inactive pre-MPF complex. Phosphorylation of cdk1/cdc2 on Thr-161 is performed by a cdk7/cyclin H complex and is necessary for activation of the cdc2 complex. Dephosphorylation of Thr-14 and Tyr-15 by CDC25 occurs at the prophase/metaphase transition and completes activation of the cdc2/cyclin B complex, initiating the cell's entry into mitosis. This antibody is routinely tested by western blot analysis.
Synonyms: Cdc2, p34

分子量

34 kDa

途径

Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA