RAS 抗体 (AA 1-190)
(5 references)Quick Overview for RAS 抗体 (AA 1-190) (ABIN967686)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-190
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交叉反应
- 小鸡, 犬, 小鼠, 大鼠
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产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Human Ras (Ha-ras) aa. 1-190
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亚型
- IgG1 kappa
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anti-RAS (RAS) (AA 1-186) antibody
RAS 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal AT2G9 unconjugated
anti-RAS (RAS) (pan) antibodyRAS 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
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说明
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Related Products: ABIN968533, ABIN967389
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 250 μg/mL
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缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store undiluted at -20° C.
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: "Regulation of Raf-1 activation and signalling by dephosphorylation." in: The EMBO journal, Vol. 21, Issue 1-2, pp. 64-71, (2002) (PubMed).
: "Thrombopoietin-mediated sustained activation of extracellular signal-regulated kinase in UT7-Mpl cells requires both Ras-Raf-1- and Rap1-B-Raf-dependent pathways." in: Molecular and cellular biology, Vol. 21, Issue 8, pp. 2659-70, (2001) (PubMed).
: "Rab5 induces Rac-independent lamellipodia formation and cell migration." in: Molecular biology of the cell, Vol. 10, Issue 10, pp. 3239-50, (1999) (PubMed).
: "The GTPase superfamily: a conserved switch for diverse cell functions." in: Nature, Vol. 348, Issue 6297, pp. 125-32, (1990) (PubMed).
: "The ras oncogene--an important regulatory element in lower eucaryotic organisms." in: Microbiological reviews, Vol. 53, Issue 2, pp. 171-85, (1989) (PubMed).
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: "Regulation of Raf-1 activation and signalling by dephosphorylation." in: The EMBO journal, Vol. 21, Issue 1-2, pp. 64-71, (2002) (PubMed).
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- RAS
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别名
- Ras
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背景
- Ras and related proteins of the Ras superfamily play critical roles in the control of normal and neoplastic proliferation. In mammalian cells there are four true Ras proteins (encoded by Ha-ras, N-ras, Ki-rasA, and Ki-rasB ) which, upon mutational activation, can function as independent oncogenes. These proteins relay signals from tyrosine kinases at the plasma membrane which subsequently lead to the nucleus via a network of serine/threonine kinases . The p21ras protein is active in its GTP-bound state. This form is slowly converted to the GDP-bound form by the intrinsic GTPase activity of Ras. This activity is greatly enhanced byGTPase-activating proteins (GAPs) which subsequently lead to removal of the GTP molecule and replacement with GDP. Maintenance of Ras in the GTP form can lead to transformation. One class of Ras mutations, commonly found in human tumors, results in an accumulation of Ras-GTP. The mutant Ras can bind GAP, but GAP bound in this manner seems unable to affect the Ras-GTPase activity. This antibody is routinely tested by western blot analysis.
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分子量
- 21 kDa
抗原
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