IFNGR1 抗体
(1 reference)Quick Overview for IFNGR1 抗体 (ABIN967652)
抗原
See all IFNGR1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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品牌
- BD Pharmingen™
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产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Purified preparation of soluble recombinant mouse IFN-gammaRalpha chain protein
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亚型
- IgG1 kappa
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anti-Interferon gamma Receptor 1 (IFNGR1) (AA 18-245) antibody
IFNGR1 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 3E1A9 unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) (AA 270-489) antibodyIFNGR1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) (AA 18-245) antibodyIFNGR1 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 5H1C9 unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) (AA 1-489) antibodyIFNGR1 适用: 人 WB, FACS 宿主: 小鼠 Polyclonal unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) antibodyKO Validated IFNGR1 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) (C-Term) antibodyIFNGR1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) (AA 431-480) antibodyIFNGR1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) (AA 18-245) antibodyIFNGR1 适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) (AA 181-193) antibodyVerified IFNGR1 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Interferon gamma Receptor 1 (IFNGR1) (AA 1-489) antibodyIFNGR1 适用: 人 IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunofluorescent Staining and Flow Cytometric Analysis: The purified form of 2E2 can be used for the immunofluorescent staining (Less or equal than 1 µg antibody/10e6 cells) and flow cytometric analysis of normal mouse cells or cell lines to measure their expressed levels of IFN-gammaRalpha. An appropriate purified immunoglobulin isotype control is A19-3. A three-layer staining protocol is recommended for maximizing the detection IFN-gammaRalpha chains expressed by cells as detailed in the figure legend.
2E2 is a nonblocking antibody that can be used for the unobstructed immunofluorescent staining and flow cytometric analysis of cells in systems where the ligand (i.e., IFN-gamma) for IFN-gamma receptors is present. Immunoprecipitation: The 2E2 antibody has been reported to be useful for the immunoprecipitation of IFN-gammaRalpha chains from lysates of cloned mouse T cells. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4° C.
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: "Ligand-induced autoregulation of IFN-gamma receptor beta chain expression in T helper cell subsets." in: Science (New York, N.Y.), Vol. 270, Issue 5239, pp. 1215-8, (1996) (PubMed).
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: "Ligand-induced autoregulation of IFN-gamma receptor beta chain expression in T helper cell subsets." in: Science (New York, N.Y.), Vol. 270, Issue 5239, pp. 1215-8, (1996) (PubMed).
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- IFNGR1 (Interferon gamma Receptor 1 (IFNGR1))
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别名
- CD119
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背景
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The 2E2 antibody recognizes the extracellular region of the 90 kDa alpha chain subunit of the mouse interferon-gamma receptor (IFN-gammaRalpha, aka, CD119). The functionally active-form of the mouse IFN-gamma receptor consists of two (or more) subunits, with IFN-gammaRalpha responsible for IFN-gamma binding and both the IFN-gammaRalpha and IFN-gammaRbeta chains required for the transduction of biologic responses. IFN-gammaRalpha is expressed by a variety of cell lines and normal mouse cells (except mature erythrocytes) including T cells, B cells, NK cells, monocytes, neutrophils, fibroblasts, epithelial and endothelial cells. The 2E2 antibody is a non-neutralizing antibody, it does not block the binding of IFN-gamma to its receptor. The immunogen used to generate this hybridoma was a purified preparation of soluble recombinant mouse IFN-gammaRalpha chain protein.
Synonyms: IFN-gamma Receptor alpha chain -
途径
- Interferon-gamma Pathway
抗原
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