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LAT 抗体 (pTyr171) (Biotin)

Name: LAT 抗体 (pTyr171) (Biotin)
SKU: ABIN967609
Availability: OutOfStock

This anti-LAT antibody is a 小鼠单克隆 antibody detecting LAT in WB 和 IHC (f). Suitable for 人. This Primary Antibody has been cited in 6+ publications.

Western blot analysis of LAT (pY171) on human T lymphocytes. (LAT 抗体 (pTyr171) (Biotin))

1 image

(6 references)

产品编号 ABIN967609

发货至: 中国

Quick Overview for LAT 抗体 (pTyr171) (Biotin) (ABIN967609)

抗原

See all LAT 抗体

LAT (Linker For Activation of T Cells (LAT))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This LAT antibody is conjugated to Biotin

应用范围

Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

I58-1169

Discover our top product LAT Primary Antibody

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pTyr171

品牌

BD Pharmingen™

产品特性

1. Please refer to us for technical protocols.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Phosphorylated Human LAT

亚型

IgG1 kappa
anti-Linker For Activation of T Cells (LAT) antibody
LAT 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Linker For Activation of T Cells (LAT) (Cytoplasmic Domain) antibody
LAT 适用: 人 WB, IP, ICFC 宿主: 小鼠 Monoclonal LAT-01 unconjugated

anti-Linker For Activation of T Cells (LAT) (pTyr191) antibody
LAT 适用: 人 WB, ELISA, IHC, IP, IF 宿主: 兔 Monoclonal 2E4 unconjugated Recombinant Antibody

anti-Linker For Activation of T Cells (LAT) (AA 1-233) antibody
LAT 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3B11-1D5 unconjugated

anti-Linker For Activation of T Cells (LAT) (AA 30-237) antibody
LAT 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Linker For Activation of T Cells (LAT) (pTyr161) antibody
LAT 适用: 人 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Linker For Activation of T Cells (LAT) (Cytoplasmic Domain) antibody
LAT 适用: 人 WB, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal LAT1111 unconjugated

anti-Linker For Activation of T Cells (LAT) (AA 25-221) antibody
LAT 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Linker For Activation of T Cells (LAT) (AA 33-227) antibody
LAT 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Linker For Activation of T Cells (LAT) (Tyr255) antibody
LAT 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Aqueous buffered solution containing ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store undiluted at 4°C.
Cho, Velikovsky, Swaminathan, Houtman, Samelson, Mariuzza: "Structural basis for differential recognition of tyrosine-phosphorylated sites in the linker for activation of T cells (LAT) by the adaptor Gads." in: The EMBO journal, Vol. 23, Issue 7, pp. 1441-51, (2004) (PubMed).

Janssen, Zhang: "Adaptor proteins in lymphocyte activation." in: Current opinion in immunology, Vol. 15, Issue 3, pp. 269-76, (2003) (PubMed).

Samelson: "Signal transduction mediated by the T cell antigen receptor: the role of adapter proteins." in: Annual review of immunology, Vol. 20, pp. 371-94, (2002) (PubMed).

Zhu, Janssen, Zhang: "Minimal requirement of tyrosine residues of linker for activation of T cells in TCR signaling and thymocyte development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 1, pp. 325-33, (2002) (PubMed).

Lin, Weiss: "Identification of the minimal tyrosine residues required for linker for activation of T cell function." in: The Journal of biological chemistry, Vol. 276, Issue 31, pp. 29588-95, (2001) (PubMed).

Paz, Wang, Clarke, Lu, Stokoe, Abo: "Mapping the Zap-70 phosphorylation sites on LAT (linker for activation of T cells) required for recruitment and activation of signalling proteins in T cells." in: The Biochemical journal, Vol. 356, Issue Pt 2, pp. 461-71, (2001) (PubMed).
抗原

LAT (Linker For Activation of T Cells (LAT))

别名

LAT

背景

Engagement of the T cell receptor (TCR) induces signal transduction pathways that enhance gene transcription and cellular proliferation and differentiation. TCR ligation results in the recruitment and activation of multiple protein tyrosine kinases (PTKs), including lck, fyn, and ZAP70. Adaptor proteins, such as Grb2 and SLP-76, relay the signal to downstream effector molecules. LAT (linker for activation of T cells) is a substrate of the activated ZAP70 and functions to bridge the activated TCR and its associated PTKs with tyrosine kinase substrates. LAT is expressed as 36- and 38-kDa forms that result from post-translational modification, and as a 42-kDa form that results from alternative splicing. LAT is an integral membrane protein that is phosphorylated at five tyrosine sites upon TCR ligation. Following phosphorylation, LAT binds a number of important signaling molecules, including Grb2, Vav, PLCgamma1, and the p85 subunit of PI3K. Multiple studies have shown that functional LAT is required for T lymphocyte activation and thymocyte development.
The I58-1169 monoclonal antibody recognizes the phosphorylated tyrosine 171 (pY171) of LAT, which is one of the phosphotyrosine sites required for binding phosphoinositide 3-kinase, Grb2, and Gads.

分子量

38 kDa

途径

TCR Signaling, Fc-epsilon Receptor Signaling Pathway