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PDGFRB 抗体 (pTyr857)

Name: PDGFRB 抗体 (pTyr857)
SKU: ABIN967592
Availability: OutOfStock

This anti-PDGFRB antibody is a 小鼠单克隆 antibody detecting PDGFRB in WB 和 IHC (f). Suitable for 人和小鼠. This Primary Antibody has been cited in 4+ publications.

Western blot analysis of PDGFRbeta (pY857). (PDGFRB 抗体 (pTyr857))

PDGFRbeta (pY857) staining on tonsil. (PDGFRB 抗体 (pTyr857))

Cells treated with a phosphatase to eliminate all phosphorylation (PDGFRB 抗体 (pTyr857))

Western Blotting (WB) image for anti-Platelet Derived Growth Factor Receptor beta (PDGFRB) (pTyr857) antibody (ABIN967592) (PDGFRB 抗体 (pTyr857))

4 images

(4 references)

产品编号 ABIN967592

发货至: 中国

Quick Overview for PDGFRB 抗体 (pTyr857) (ABIN967592)

抗原

See all PDGFRB 抗体

PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

适用

人, 小鼠

宿主

小鼠

克隆类型

单克隆

标记

This PDGFRB antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

J24-425

Discover our top product PDGFRB Primary Antibody

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pTyr857

品牌

BD Pharmingen™

交叉反应

小鼠

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Phosphorylated Human PDGFRbeta

亚型

IgG1 kappa
anti-Platelet Derived Growth Factor Receptor beta (PDGFRB) (AA 33-133) antibody
PDGFRB 适用: 人 WB, ELISA, IP, PLA 宿主: 小鼠 Monoclonal 4C12 unconjugated

anti-Platelet Derived Growth Factor Receptor beta (PDGFRB) (AA 887-1106) antibody
PDGFRB 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Platelet Derived Growth Factor Receptor beta (PDGFRB) (AA 981-1066) antibody
PDGFRB 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Platelet Derived Growth Factor Receptor beta (PDGFRB) (AA 1-1106) antibody
PDGFRB 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

anti-Platelet Derived Growth Factor Receptor beta (PDGFRB) (AA 40-72) antibody
PDGFRB 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-Platelet Derived Growth Factor Receptor beta (PDGFRB) (AA 33-133) antibody
PDGFRB 适用: 人, 小鼠 ELISA, FACS 宿主: 小鼠 Monoclonal 2G7B7 unconjugated

anti-Platelet Derived Growth Factor Receptor beta (PDGFRB) antibody
PDGFRB 适用: 人 WB, IF 宿主: 小鼠 Monoclonal 1G9 unconjugated

anti-Platelet Derived Growth Factor Receptor beta (PDGFRB) (AA 1-1106) antibody
PDGFRB 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-Platelet Derived Growth Factor Receptor beta (PDGFRB) antibody
Microarray verified PDGFRB 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB472367 unconjugated

anti-Platelet Derived Growth Factor Receptor beta (PDGFRB) (AA 718-767) antibody
PDGFRB 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

说明

Related Products: ABIN967389

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Aqueous buffered solution containing ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store undiluted at 4°C.
Chiarugi, Cirri, Taddei, Giannoni, Fiaschi, Buricchi, Camici, Raugei, Ramponi: "Insight into the role of low molecular weight phosphotyrosine phosphatase (LMW-PTP) on platelet-derived growth factor receptor (PDGF-r) signaling. LMW-PTP controls PDGF-r kinase activity through TYR-857 dephosphorylation." in: The Journal of biological chemistry, Vol. 277, Issue 40, pp. 37331-8, (2002) (PubMed).

Baxter, Secrist, Vaillancourt, Kazlauskas: "Full activation of the platelet-derived growth factor beta-receptor kinase involves multiple events." in: The Journal of biological chemistry, Vol. 273, Issue 27, pp. 17050-5, (1998) (PubMed).

Liu, Oh, Horner, Rogers, Schnitzer: "Organized endothelial cell surface signal transduction in caveolae distinct from glycosylphosphatidylinositol-anchored protein microdomains." in: The Journal of biological chemistry, Vol. 272, Issue 11, pp. 7211-22, (1997) (PubMed).

Claesson-Welsh: "Platelet-derived growth factor receptor signals." in: The Journal of biological chemistry, Vol. 269, Issue 51, pp. 32023-6, (1995) (PubMed).
抗原

PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

别名

CD140b

背景

Platelet-derived growth factor (PDGF) is a potent mitogen for cells of mesenchymal origin and exerts its effects by binding to the PDGF receptor (PDGFR), a transmembrane protein tyrosine kinase. PDGFR is composed of PDGFRalpha (CD140a) and/or PDGFRbeta (CD140b) polypeptides. Both PDGF and PDGFR consist of subunits that form homo- or heterodimers with varying specificities: PDGF-AA binds only to alphaalpha PDGFR, PDGF-AB binds to both alphaalpha and alphabeta PDGFR, and PDGF-BB binds to all three PDGFRs. Ligand binding induces dimerization and activation of the receptor. Upon activation, CD140b is phosphorylated at multiple tyrosine sites and, in turn, an intracellular phosphorylation cascade is initiated. PDGFR localizes primarily to membrane invaginations termed caveolae, compartments that are enriched in several of its downstream effectors, including phosphatidylinositol 3'-kinase, Src, and phospholipase C-gamma.
The J24-425 monoclonal antibody recognizes the phosphorylated tyrosine 857 (pY857) in the tyrosine kinase domain 2 of CD140b, which is required for maximal receptor kinase activity. The orthologous phosphorylation site in mouse PDGFRbeta is Y856.

分子量

180 kDa

途径

Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling