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GFAP 抗体

GFAP 适用: 人, 大鼠, 小鼠, Pig, Cow, 小鸡, 兔, 豚鼠, 绵羊, 犬 WB, IF, IHC (f) 宿主: 小鼠 Monoclonal 4A11 unconjugated
产品编号 ABIN967499
发货至: 中国
  • 抗原 See all GFAP 抗体
    GFAP (Glial Fibrillary Acidic Protein (GFAP))
    适用
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    人, 大鼠, 小鼠, Pig, Cow, 小鸡, 兔, 豚鼠, 绵羊, 犬
    宿主
    • 184
    • 139
    • 12
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    小鼠
    克隆类型
    • 185
    • 156
    单克隆
    标记
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    This GFAP antibody is un-conjugated
    应用范围
    • 274
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    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    品牌
    BD Pharmingen™
    交叉反应
    人, 小鼠, 大鼠, 绵羊, 犬, 猪, 兔, 豚鼠, 小鸡
    产品特性
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    纯化方法
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    免疫原
    Cow spinal cord homogenate
    克隆位点
    4A11
    亚型
    IgG2b
    Top Product
    Discover our top product GFAP Primary Antibody
  • 应用备注
    Applications include immunohistochemical staining of formalin-fixed paraffin-embedded brain tissue sections (25 µg/ml). Other applications not routinely tested include indirect immunofluorescence of tissue-cultured cells and western blot analysis (1-2 µg/ml). Rat brain is suggested as a positive control.
    说明

    Related Products: ABIN967501, ABIN967500, ABIN967502

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store undiluted at 4°C.
  • Schwartz, Cheng, Mughal, Mattson, Yao: "Clathrin assembly proteins AP180 and CALM in the embryonic rat brain." in: The Journal of comparative neurology, Vol. 518, Issue 18, pp. 3803-18, (2010) (PubMed).

    Graziano, Liu, Murray, Jones: "Vesicular glutamate transporters define two sets of glutamatergic afferents to the somatosensory thalamus and two thalamocortical projections in the mouse." in: The Journal of comparative neurology, Vol. 507, Issue 2, pp. 1258-76, (2008) (PubMed).

    McLendon, Bigner: "Immunohistochemistry of the glial fibrillary acidic protein: basic and applied considerations." in: Brain pathology (Zurich, Switzerland), Vol. 4, Issue 3, pp. 221-8, (1994) (PubMed).

    McLendon, Burger, Pegram, Eng, Bigner: "The immunohistochemical application of three anti-GFAP monoclonal antibodies to formalin-fixed, paraffin-embedded, normal and neoplastic brain tissues." in: Journal of neuropathology and experimental neurology, Vol. 45, Issue 6, pp. 692-703, (1986) (PubMed).

    Pegram, Eng, Wikstrand, McComb, Lee, Bigner: "Monoclonal antibodies reactive with epitopes restricted to glial fibrillary acidic proteins of several species." in: Neurochemical pathology, Vol. 3, Issue 2, pp. 119-38, (1985) (PubMed).

  • 抗原
    GFAP (Glial Fibrillary Acidic Protein (GFAP))
    别名
    GFAP (GFAP 产品)
    别名
    GFAP antibody, AI836096 antibody, cb345 antibody, etID36982.3 antibody, gfapl antibody, wu:fb34h11 antibody, wu:fk42c12 antibody, xx:af506734 antibody, zgc:110485 antibody, glial fibrillary acidic protein antibody, GFAP antibody, LOC100136168 antibody, gfap antibody, Gfap antibody
    背景
    GFAP (Glial Fibrillary Acid Protein) is the major protein of glial filaments in differentiated astrocytes. We offer a panel of monoclonal antibodies (4A11, 1B4, 2E1) that specifically recognize GFAP. They do not cross-react with other intermediate filaments such as vimentin, neurofilament proteins, desmin, keratin, neurotubules or microfilaments. Clone 4A11 is specific for GFAP. Bovine spinal cord homogenate was used as immunogen. This antibody has broad species reactivity, recognizing GFAP in brain homogenates from human, mouse, rat, bovine, ovine, canine, porcine, rabbit, guinea pig and chicken. 4A11 is particularly useful for identifying GFAP in immunohistochemistry of frozen and formalin-fixed, paraffin-embedded brain tissue sections. Additional applications include western blot analysis and indirect immunofluorescence of tissue-cultured cells. We offer additional GFAP specific antibodies: clone 2E1 (ABIN967501), 1B4 (ABIN967500), and clones 1B4, 4A11, 2E1 combined and available as a cocktail” (ABIN967502).
    分子量
    50 kDa
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