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TUBB 抗体

Name: TUBB 抗体
SKU: ABIN967495
Availability: OutOfStock

This anti-TUBB antibody is a 小鼠单克隆 antibody detecting TUBB in WB, IHC 和 BI. Suitable for 人, 小鼠, 大鼠和 Cow. This Primary Antibody has been cited in 5+ publications.

Immunofluorescent staining of U-87 MG (ATCC HTB-14) cells. (TUBB 抗体)

Image no. 2 for anti-Tubulin, beta (TUBB) antibody (ABIN967495) (TUBB 抗体)

2 images

(5 references)

产品编号 ABIN967495

发货至: 中国

Quick Overview for TUBB 抗体 (ABIN967495)

抗原

See all TUBB 抗体

TUBB (Tubulin, beta (TUBB))

适用

人, 小鼠, 大鼠, Cow

宿主

小鼠

克隆类型

单克隆

标记

This TUBB antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), BioImaging (BI)

克隆位点

5H1

Discover our top product TUBB Primary Antibody

品牌

BD Pharmingen™

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
3. Triton is a trademark of the Dow Chemical Company.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Please refer to us for technical protocols.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

亚型

IgM kappa
anti-Tubulin, beta (TUBB) antibody
TUBB 适用: 人, 小鼠, 大鼠, Pig, 小鸡, Tetrahymena, 鱼, Nicotiana tabacum, Arabidopsis, Paramecium WB, ICC, IHC (p) 宿主: 小鼠 Monoclonal TU-06 unconjugated

anti-Tubulin, beta (TUBB) antibody
TUBB 适用: 人 WB, IF, ICC, IHC (p), IHC (wm) 宿主: 兔 Polyclonal unconjugated

anti-Tubulin, beta (TUBB) (AA 61-160) antibody
TUBB 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Tubulin, beta (TUBB) (AA 170-419) antibody
TUBB 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tubulin, beta (TUBB) (AA 170-419) antibody
TUBB 适用: 人 WB 宿主: 小鼠 Monoclonal C3 unconjugated

anti-Tubulin, beta (TUBB) (AA 170-419) antibody
TUBB 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C6 unconjugated

anti-Tubulin, beta (TUBB) (AA 1-444) antibody
TUBB 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3B3 unconjugated

anti-Tubulin, beta (TUBB) (AA 160-447) antibody
TUBB 适用: 人, 小鼠 WB, IHC, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Tubulin, beta (TUBB) antibody
TUBB 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 87CT59-3-7 unconjugated

anti-Tubulin, beta (TUBB) (AA 1-50) antibody
TUBB 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.

说明

Related Products: ABIN967389

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Aqueous buffered solution containing ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store undiluted at 4°C.
Cho, Johnson: "Primed phosphorylation of tau at Thr231 by glycogen synthase kinase 3beta (GSK3beta) plays a critical role in regulating tau's ability to bind and stabilize microtubules." in: Journal of neurochemistry, Vol. 88, Issue 2, pp. 349-58, (2003) (PubMed).

Helfand, Mikami, Vallee, Goldman: "A requirement for cytoplasmic dynein and dynactin in intermediate filament network assembly and organization." in: The Journal of cell biology, Vol. 157, Issue 5, pp. 795-806, (2002) (PubMed).

Prahlad, Yoon, Moir, Vale, Goldman: "Rapid movements of vimentin on microtubule tracks: kinesin-dependent assembly of intermediate filament networks." in: The Journal of cell biology, Vol. 143, Issue 1, pp. 159-70, (1998) (PubMed).

Brown, Wood, Cleveland: "The kinesin-like protein CENP-E is kinetochore-associated throughout poleward chromosome segregation during anaphase-A." in: Journal of cell science, Vol. 109 ( Pt 5), Issue 4, pp. 961-9, (1996) (PubMed).

Wang, Loomis, Zinkowski, Binder: "A novel tau transcript in cultured human neuroblastoma cells expressing nuclear tau." in: The Journal of cell biology, Vol. 121, Issue 2, pp. 257-67, (1993) (PubMed).
抗原

TUBB (Tubulin, beta (TUBB))

别名

beta-Tubulin

背景

Tubulin is a highly conserved protein with a molecular weight of ~50 kD. The self-assembly of tubulin leads to microtubules, hollow cylinders that are one of the major components of the eukaryotic cytoskeleton. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. There are two main classes of tubulin isoforms, alpha- and beta-tubulin, which are usually products of separate genes. Microtubules are made from protofilaments, strings of alternating alpha- and beta-tubulin spaced 4 nm apart and pointing in the same direction. Tubulin can be posttranslationally modified in several ways, including phosphorylation, acetylation, glutamylation, and detyrosination. For example, microtubules that turn over slowly tend to be acetylated and detyrosinated.
The 5H1 monoclonal antibody reacts with beta-tubulin. It does not cross-react with alpha-tubulin.

分子量

50 kDa

途径

Microtubule Dynamics, M Phase