CD9 抗体
Quick Overview for CD9 抗体 (ABIN967484)
抗原
See all CD9 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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品牌
- BD Pharmingen™
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产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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亚型
- IgG1 kappa
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应用备注
- This reagent is effective for indirect immunofluorescence staining of human cells for flow cytometric analysis. It also worked by western blotting with the human platelets lysate at the concentration of 2-4 µg/ml (in-house data). It also can be used for immunohistochemistry on frozen tissue (spleen/thymus) sections at 2-10 µg/ml (in-house data). However, we do not recommend using this antibody on the formalin fixed paraffin tissue sections.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4° C.
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: "CD9-regulated adhesion. Anti-CD9 monoclonal antibody induce pre-B cell adhesion to bone marrow fibroblasts through de novo recognition of fibronectin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 6, pp. 2768-77, (1994) (PubMed).
: "Platelet alpha-granule and plasma membrane share two new components: CD9 and PECAM-1." in: Blood, Vol. 84, Issue 6, pp. 1722-30, (1994) (PubMed).
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: "CD9-regulated adhesion. Anti-CD9 monoclonal antibody induce pre-B cell adhesion to bone marrow fibroblasts through de novo recognition of fibronectin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 6, pp. 2768-77, (1994) (PubMed).
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- CD9
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别名
- CD9
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背景
- Reacts with a 24 kDa type III transmembrane protein which is expressed on platelets, pre-B cells, monocytes, endothelia, and epithelia. CD9 belongs to a family of membrane proteins called tetraspanins which transverse the membrane four times. CD9 is weakly expressed on resting mature B cells. M-L13 induces platelet aggregation and activation. This antibody is also suitable for staining acetone-fixed, frozen tissue sections.
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途径
- Response to Water Deprivation, Cell-Cell Junction Organization
抗原
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