GM-CSF 抗体
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北京 101111
Quick Overview for GM-CSF 抗体 (ABIN967465)
抗原
See all GM-CSF (CSF2) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Ficoll-Paque is a trademark of Amersham Biosciences Limited.
5. Cy is a trademark of Amersham Biosciences Limited. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Recombinant human GM-CSF
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亚型
- IgG2a
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anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 71-141) antibody
CSF2 适用: Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 65-144) antibodyCSF2 适用: 人 WB, ELISA, IF, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) antibodyCSF2 适用: 小鼠 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) antibodyCSF2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 59-85) antibodyCSF2 适用: 人 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal RB23360 unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 19-141) antibodyCSF2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) antibodyCSF2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) antibodyCSF2 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-141) antibodyCSF2 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 62-89), (Middle Region) antibodyCSF2 适用: 人 WB, FACS, IF, EIA, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Blocking Control for Intracellular Staining:
The purified BVD2-21C11 antibody (ABIN967465) can be used as a blocking control to demonstrate specificity of human GM-CSF staining by the PE-BVD2-21C11. To perform this control, the fixed/permeabilized cells (~ 1 million) can be incubated with 1 - 10 µg of unlabeled BVD2-21C11 antibody (ABIN967465) for 20 minutes at 4°C, prior to staining with PE-BVD2-21C11 antibody (eg, 0.1 - 0.5 µg mAb/ 1 million cells). The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.
ELISA Detection:
The biotinylated BVD2-21C11 antibody, is useful as a detection antibody for a sandwich ELISA for measuring human GM-CSF protein levels. Biotinylated BVD2-21C11 antibody can be paired with the purified BVD2-23B6 antibody as the capture antibody, with recombinant human GM-CSF as the standard. This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).
: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).
: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).
: "Granulocyte/macrophage colony-stimulating factor and interleukin 3 release from human peripheral blood eosinophils and neutrophils." in: The Journal of experimental medicine, Vol. 174, Issue 3, pp. 745-8, (1991) (PubMed).
: "Host-reactive CD4+ and CD8+ T cell clones isolated from a human chimera produce IL-5, IL-2, IFN-gamma and granulocyte/macrophage-colony-stimulating factor but not IL-4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 3, pp. 902-8, (1990) (PubMed).
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: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).
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- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
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别名
- GM-CSF
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背景
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The BVD2-21C11 monoclonal antibody specifically binds to human Granulocyte/Macrophage - Colony Stimulating Factor (GM-CSF). Human GM-CSF is encoded by the CSF2 gene and is also known as Colony Stimulating Factor 2. GM-CSF is produced by activated T lymphocytes, macrophages, endothelial cells, fibroblasts, stromal cells and other cell types including B lymphocytes, mast cells, eosinophils, and osteoblasts. GM-CSF stimulates the survival, proliferation and/or differentiation of various cell types including neutrophils, eosinophils, macrophages, dendritic cells, megakaryocytes, erythroid cells, endothelial cells and their precursors. The immunogen used to generate the BVD2-21C11 hybridoma was recombinant human GM-CSF. The BVD2-21C11 antibody has been reported to crossreact with GM-CSF from the rhesus monkey. BVD2-21C11 is a neutralizing antibody.
Synonyms: CSF2, Colony stimulating factor 2 (granulocyte-macrophage), CSF, GMCSF -
途径
- JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin
抗原
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