(5 references)-
- 抗原 See all SPI1 抗体
- SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This SPI1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP)
- 品牌
- BD Pharmingen™
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- PU.1 Fusion Protein
- 克隆位点
- G148-74
- 亚型
- IgG2a
- Top Product
- Discover our top product SPI1 Primary Antibody
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anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 16-170) antibody
SPI1 适用: 人 WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal PU1-2146 unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 124-271) antibodySPI1 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 5C7 unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 1-120) antibodySPI1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2G1 unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (Internal Region) antibodyVerified SPI1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 1-122) antibodySPI1 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 217-246) antibodySPI1 适用: 人 WB, IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 1-264) antibodySPI1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 20-70) antibodySPI1 适用: 人, 小鼠, 犬, Cow, 马, 猴 WB, FACS 宿主: 兔 Polyclonal unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 18-196) antibodySPI1 适用: 人, 小鼠, 大鼠 IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) (AA 217-246), (C-Term) antibodySPI1 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Applications include gel shift, western blot analysis (1-2 µg/ml) and immunoprecipitation. The antibody has been shown to recognize in vitro translated PU.1 and PU.1 expressed as a recombinant protein in bacteria. In. In gel shift assays using µB as a probe (µB is a transcriptional element in the immunoglobulin heavy chain enhancer), G148-74 supershifts the complex of probe and PU.1.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Pillars article: the macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene. Cell, 1990. 61: 113-124." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 3, pp. 1597-608, (2008) (PubMed).
: "Ets proteins: new factors that regulate immunoglobulin heavy-chain gene expression." in: Molecular and cellular biology, Vol. 13, Issue 11, pp. 7163-9, (1993) (PubMed).
: "Ets-related protein PU.1 regulates expression of the immunoglobulin J-chain gene through a novel Ets-binding element." in: Genes & development, Vol. 7, Issue 10, pp. 2006-15, (1993) (PubMed).
: "PU.1 recruits a second nuclear factor to a site important for immunoglobulin kappa 3' enhancer activity." in: Molecular and cellular biology, Vol. 12, Issue 1, pp. 368-78, (1992) (PubMed).
: "The putative oncogene Spi-1: murine chromosomal localization and transcriptional activation in murine acute erythroleukemias." in: Oncogene, Vol. 4, Issue 12, pp. 1449-56, (1990) (PubMed).
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: "Pillars article: the macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene. Cell, 1990. 61: 113-124." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 3, pp. 1597-608, (2008) (PubMed).
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- 抗原
- SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
- 别名
- PU.1 (SPI1 产品)
- 别名
- OF antibody, PU.1 antibody, SFPI1 antibody, SPI-1 antibody, SPI-A antibody, Dis-1 antibody, Dis1 antibody, Sfpi-1 antibody, Sfpi1 antibody, Spi-1 antibody, Tcfpu1 antibody, Tfpu.1 antibody, Pu.1 antibody, cb1086 antibody, pu.1 antibody, pu1 antibody, sfpi1 antibody, si:by184l24.2 antibody, spi1 antibody, SPI1/PU.1 antibody, spi1l antibody, zgc:152991 antibody, Spi-1 proto-oncogene antibody, spleen focus forming virus (SFFV) proviral integration oncogene antibody, Spi-1 proto-oncogene b antibody, Spi-1 proto-oncogene a antibody, SPI1 antibody, Spi1 antibody, spi1b antibody, spi1a antibody
- 背景
- PU.1 is an ets-related transcription factor expressed in B lymphocytes and macrophages. It was initially identified as the oncogene Spi-1 which was found to block erythroblast differentiation. PU.1 functions by binding transcriptional control elements such as µB in the immunoglobulin heavy chain enhancer and pi adjacent to µE2. It has been shown to form a complex with NF-EM5, a B cell transcription factor, resulting in the activation of the immunoglobulin kappa 3' enhancer. PU.1 also appears to be critically involved in the control of monocyte development by regulating the expression of the macrophage colony-stimulating factor receptor. PU.1 runs as a 40-42 kDa protein on SDS-PAGE. G148-74 recognizes human PU.1, an Ets-related transcription factor. PU.1 is expressed in B lymphocytes and macrophages and like other Ets-related proteins, binds to consensus sites in DNA through an 85 amino acid Ets domain in the carboxyl terminal region of the protein. The antibody was raised against a bacterially expressed GST-PU.1 fusion protein.
- 分子量
- 40-42 kDa
- 途径
- Stem Cell Maintenance
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