Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) 抗体

ABIN967452 产品详细信息, 供应商: Log in to see
抗原
  • OF
  • PU.1
  • SFPI1
  • SPI-1
  • SPI-A
  • Dis-1
  • Dis1
  • Sfpi-1
  • Sfpi1
  • Spi-1
  • Tcfpu1
  • Tfpu.1
  • Pu.1
  • cb1086
  • pu.1
  • pu1
  • sfpi1
  • si:by184l24.2
  • spi1
  • SPI1/PU.1
  • Spi-1 proto-oncogene
  • spleen focus forming virus (SFFV) proviral integration oncogene
  • Spi-1 proto-oncogene b
  • SPI1
  • Spi1
  • spi1b
适用
人
146
38
27
13
12
11
10
6
5
5
5
4
2
宿主
小鼠
92
33
20
6
克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
6
6
6
6
4
3
3
3
2
2
2
2
2
1
应用范围
Immunoprecipitation (IP), Western Blotting (WB)
108
57
36
26
22
15
2
2
2
2
2
2
2
1
选项
Supplier
Log in to see
Supplier Product No.
Log in to see
品牌 BD Pharmingen™
免疫原 PU.1 Fusion Protein
克隆位点 G148-74
亚型 IgG2a
产品特性 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
纯化方法 The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
别名 PU.1 (SPI1 Antibody 摘要)
背景 PU.1 is an ets-related transcription factor expressed in B lymphocytes and macrophages. It was initially identified as the oncogene Spi-1 which was found to block erythroblast differentiation. PU.1 functions by binding transcriptional control elements such as µB in the immunoglobulin heavy chain enhancer and pi adjacent to µE2. It has been shown to form a complex with NF-EM5, a B cell transcription factor, resulting in the activation of the immunoglobulin kappa 3' enhancer. PU.1 also appears to be critically involved in the control of monocyte development by regulating the expression of the macrophage colony-stimulating factor receptor. PU.1 runs as a 40-42 kDa protein on SDS-PAGE. G148-74 recognizes human PU.1, an Ets-related transcription factor. PU.1 is expressed in B lymphocytes and macrophages and like other Ets-related proteins, binds to consensus sites in DNA through an 85 amino acid Ets domain in the carboxyl terminal region of the protein. The antibody was raised against a bacterially expressed GST-PU.1 fusion protein.
分子量 40-42 kDa
途径 Stem Cell Maintenance
应用备注 Applications include gel shift, western blot analysis (1-2 µg/ml) and immunoprecipitation. The antibody has been shown to recognize in vitro translated PU.1 and PU.1 expressed as a recombinant protein in bacteria. In. In gel shift assays using µB as a probe (µB is a transcriptional element in the immunoglobulin heavy chain enhancer), G148-74 supershifts the complex of probe and PU.1.
限制 仅限研究用
状态 Liquid
浓度 0.5 mg/mL
缓冲液 Aqueous buffered solution containing ≤0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
储存方法 Store undiluted at 4°C.
有引用在： Klemsz, McKercher, Celada, Van Beveren, Maki: "Pillars article: the macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene. Cell, 1990. 61: 113-124." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 3, pp. 1597-608, 2008 (PubMed).

Rivera, Stuiver, Steenbergen, Murre: "Ets proteins: new factors that regulate immunoglobulin heavy-chain gene expression." in: Molecular and cellular biology, Vol. 13, Issue 11, pp. 7163-9, 1993 (PubMed).

Shin, Koshland: "Ets-related protein PU.1 regulates expression of the immunoglobulin J-chain gene through a novel Ets-binding element." in: Genes & development, Vol. 7, Issue 10, pp. 2006-15, 1993 (PubMed).

Pongubala, Nagulapalli, Klemsz, McKercher, Maki, Atchison: "PU.1 recruits a second nuclear factor to a site important for immunoglobulin kappa 3' enhancer activity." in: Molecular and cellular biology, Vol. 12, Issue 1, pp. 368-78, 1992 (PubMed).

Moreau-Gachelin, Ray, Mattei, Tambourin, Tavitian: "The putative oncogene Spi-1: murine chromosomal localization and transcriptional activation in murine acute erythroleukemias." in: Oncogene, Vol. 4, Issue 12, pp. 1449-56, 1990 (PubMed).

背景 Zhang, Hetherington, Chen, Tenen: "The macrophage transcription factor PU.1 directs tissue-specific expression of the macrophage colony-stimulating factor receptor." in: Molecular and cellular biology, Vol. 14, Issue 1, pp. 373-81, 1994 (PubMed).

Klemsz, McKercher, Celada, Van Beveren, Maki: "The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene." in: Cell, Vol. 61, Issue 1, pp. 113-24, 1990 (PubMed).

您还需要查找其他产品吗？