FLI1 抗体
(2 references)Quick Overview for FLI1 抗体 (ABIN967450)
抗原
See all FLI1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
品牌
- BD Pharmingen™
-
产品特性
-
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
-
免疫原
- Fli-1 ets Domain Fusion Protein
-
亚型
- IgG2b
-
-
anti-Friend Leukemia Virus Integration 1 (FLI1) (AA 303-452) antibody
FLI1 适用: 人 ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 4F2A9 unconjugated
anti-Friend Leukemia Virus Integration 1 (FLI1) (AA 1-452) antibodyFLI1 适用: 人 WB, ELISA, IF, RNAi 宿主: 小鼠 Monoclonal 1F6 unconjugated
anti-Friend Leukemia Virus Integration 1 (FLI1) (AA 205-331) antibodyFLI1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Friend Leukemia Virus Integration 1 (FLI1) (AA 1-452) antibodyFLI1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1G8 unconjugated
anti-Friend Leukemia Virus Integration 1 (FLI1) (AA 54-73), (N-Term) antibodyFLI1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Friend Leukemia Virus Integration 1 (FLI1) (N-Term) antibodyFLI1 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Friend Leukemia Virus Integration 1 (FLI1) (Internal Region) antibodyFLI1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Friend Leukemia Virus Integration 1 (FLI1) (Internal Region) antibodyFLI1 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Friend Leukemia Virus Integration 1 (FLI1) (AA 1-196) antibodyFLI1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Friend Leukemia Virus Integration 1 (FLI1) (Internal Region) antibodyFLI1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- G146-222 has been shown to recognize in vitro translated, recombinant bacterially expressed and endogenous B cell Fli-1. In gel shift assays, this antibody disrupts complexes of Fli-1 and DNA probe, however, this application is not tested.
-
说明
-
Related Products: ABIN967389
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.5 mg/mL
-
缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
储存方法
- Store undiluted at 4°C.
-
-
-
: "DNA-binding and transcriptional activation properties of the EWS-FLI-1 fusion protein resulting from the t(11;22) translocation in Ewing sarcoma." in: Molecular and cellular biology, Vol. 14, Issue 5, pp. 3230-41, (1994) (PubMed).
: "The Ewing's sarcoma EWS/FLI-1 fusion gene encodes a more potent transcriptional activator and is a more powerful transforming gene than FLI-1." in: Molecular and cellular biology, Vol. 13, Issue 12, pp. 7393-8, (1994) (PubMed).
-
: "DNA-binding and transcriptional activation properties of the EWS-FLI-1 fusion protein resulting from the t(11;22) translocation in Ewing sarcoma." in: Molecular and cellular biology, Vol. 14, Issue 5, pp. 3230-41, (1994) (PubMed).
-
- FLI1 (Friend Leukemia Virus Integration 1 (FLI1))
-
别名
- Fli-1
-
背景
- Fli-1 is a 50 kDa ets-related transcription factor. Like other ets-related proteins it binds to consensus sites in DNA through an 85 amino acid ets domain in the carboxyl region of the protein. Fli-1 is associated with Ewing sarcoma (EWS) through a t(11,22)(q24,q12) chromosomal translocation. This translocation brings the 5' region of the EWS gene into conjunction with the 3' region of the Fli-1 gene encoding the ets-DNA binding domain. Such a translocation is found in 85% of Ewing sarcomas. The resulting fusion protein has the DNA binding activity of Fli- 1 and, with the EWS domain, it is also a more potent transcriptional activator than Fli-1 itself. The strong transforming activity of the EWS-Fli-1 fusion protein may in part be due to its ability to transactivate the promoter for c-myc. G146-222 recognizes human Fli-1. The antibody was raised against a bacterially expressed Fli-1 ets domain fusion protein.
-
分子量
- 50 kDa
抗原
-
