CD247 抗体 (C-Term)
(1 reference)Quick Overview for CD247 抗体 (C-Term) (ABIN967446)
抗原
See all CD247 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- C-Term
-
品牌
- BD Pharmingen™
-
产品特性
-
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
-
免疫原
- Mouse CD3zeta C-terminus synthetic peptide
-
亚型
- IgG2b
-
-
anti-CD247 Molecule (CD247) (pTyr72) antibody
CD247 适用: 人, 小鼠 WB, ICC, ICFC 宿主: 小鼠 Monoclonal EM-26 unconjugated
anti-CD247 Molecule (CD247) (AA 1-164) antibodyCD247 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-CD247 Molecule (CD247) (AA 1-164) antibodyCD247 适用: 人 WB, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal 4A12-F6 unconjugated
anti-CD247 Molecule (CD247) (AA 88-153) antibodyCD247 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-CD247 Molecule (CD247) (AA 50-164) antibodyCD247 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD247 Molecule (CD247) antibodyCD247 适用: 人 ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 4B10 unconjugated
anti-CD247 Molecule (CD247) antibodyCD247 适用: 人 WB, IHC, FACS 宿主: 兔 Monoclonal unconjugated
anti-CD247 Molecule (CD247) (AA 1-165) antibodyCD247 适用: 人 WB, IP, IHC (p), EIA 宿主: 小鼠 Monoclonal 4A12-F6 unconjugated
anti-CD247 Molecule (CD247) (AA 51-100) antibodyCD247 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD247 Molecule (CD247) (C-Term) antibodyCD247 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- Applications include western blot analysis (1-2 µg/ml) and immunoprecipitation (1-2 µg/1x10 [6] cells). Immunoprecipitation application is not routinely tested, only tested during development.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.5 mg/mL
-
缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
储存方法
- Store undiluted at 4°C.
-
-
-
: "Overexpression of CD3 eta during thymic development does not alter the negative selection process." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 4, pp. 1183-94, (1993) (PubMed).
-
: "Overexpression of CD3 eta during thymic development does not alter the negative selection process." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 4, pp. 1183-94, (1993) (PubMed).
-
- CD247 (CD247 Molecule (CD247))
-
别名
- CD247
-
背景
-
The T-cell receptor (TCR), expressed by thymus-derived lymphocytes, is a multicomponent complex responsible for recognizing antigen in the context of MHC molecules. Ti is the antigen-specific binding component of the TCR. On the majority of peripheral T lymphocytes, Ti is present as a disulfide-linked alpha-beta heterodimer. A minor fraction express Ti as a gamma-delta heterodimer. The Ti subunits are Ig-like, each containing V and C regions. Ti is noncovalently associated with an invariant set of molecules referred to as the CD3 subunits. CD3 appears early in thymocyte differentiation and remains expressed on all mature T lymphocytes. In the mouse, it consists of five chains: gamma, delta, epsilon, zeta, and eta. The relative masses of the mouse CD3 chains are 21, 28, 26, 32, (dimer) and 21 kDa, respectively. Clone 1zeta3A1 recognizes an epitope in the C-terminus of mouse CD3zeta. A synthetic peptide corresponding to the C-terminus of mouse CD3zeta (BSACDTYDALHMQTLAPR) was used as immunogen. Hybridomas were derived from Balb/c spleen cells x NS-1.
Synonyms: CD3zeta chain -
分子量
- 32 kDa
-
途径
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
抗原
-
