Syndecan 1 抗体
Quick Overview for Syndecan 1 抗体 (ABIN967377)
抗原
See all Syndecan 1 (SDC1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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品牌
- BD Pharmingen™
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产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- NAMRU mouse mammary gland epithelial cell line NMuMG
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亚型
- IgG2a kappa
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anti-Syndecan 1 (SDC1) (AA 28-171) antibody
SDC1 适用: 人, 小鼠 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 1A3H4 unconjugated
anti-Syndecan 1 (SDC1) (AA 210-238), (C-Term) antibodySDC1 适用: 人 WB, FACS, IF 宿主: 小鼠 Monoclonal 587CT7-3-6-5 unconjugated
anti-Syndecan 1 (SDC1) (AA 23-253) antibodySDC1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Syndecan 1 (SDC1) (AA 58-313) antibodySDC1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Syndecan 1 (SDC1) (AA 201-310) antibodySDC1 适用: 人, 大鼠, 小鼠, Cow WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Syndecan 1 (SDC1) (AA 25-240) antibodySDC1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Syndecan 1 (SDC1) (AA 23-254) antibodySDC1 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Syndecan 1 (SDC1) (C-Term) antibodySDC1 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Syndecan 1 (SDC1) (AA 23-254) antibodySDC1 适用: 人 ELISA, FACS 宿主: 兔 Monoclonal DM45 unconjugated Recombinant Antibody
anti-Syndecan 1 (SDC1) (AA 25-240) antibodySDC1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- CD138 is expressed at low density on cells in the normal bone marrow, therefore, it may be desirable to amplify staining by using a biotinylated second-step antibody followed by a bright third-step reagent, such as Streptavidin-PE. Other reported applications include western blot analysis using a cationic blotting membrane and a specific method, ELISA, and immunohistochemical staining of acetone-fixed frozen and paraffin-embedded sections.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.
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- Syndecan 1 (SDC1)
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别名
- CD138
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背景
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The 281-2 antibody reacts with the core protein of CD138 (Syndecan-1), a cell-surface, integral membrane heparan sulfate- and chondroitin sulfate-containing proteoglycan that binds to interstitial extracellular matrix molecules. Syndecan-1 is predominantly expressed on epithelial cells, where its expression correlates with normal epithelial organization. It is also expressed on B lymphocytes at specific stages during their differentiation: precursor B cells in the bone marrow and antibody-secreting cells, including plasma cells, but not mature peripheral B cells. It is thus implicated in mediating B cell-matrix interactions. CD138 expression is also regulated during embryonic development, and the molecule shows a tissue- specific structural polymorphism resulting from different post-translational modifications. The 281-2 antibody may be used to detect the differently glycosylated forms, because it reacts with the core protein. Furthermore, the mAb detects the Syndecan-1 ectodomain which is cleaved from cell surfaces by a metalloproteinase. This antibody is routinely tested by flow cytometric analysis.
Synonyms: Syndecan-1 -
途径
- Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism
抗原
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