TER-119/Erythroid Cells 抗体
There are 2+ publications for this product available. The 大鼠 单克隆 anti-TER-119/Erythroid Cells antibody is suitable to detect TER-119/Erythroid Cells in samples from 小鼠. It has been validated for IHC (fro), FACS, IP, WB, CyTox 和 IHC (p).
(2 references)
产品编号 ABIN967376
发货至:
中国
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Quick Overview for TER-119/Erythroid Cells 抗体 (ABIN967376)
抗原
TER-119/Erythroid Cells
适用
小鼠
宿主
大鼠
克隆类型
单克隆
应用范围
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Cytotoxicity Test (CyTox), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
克隆位点
TER-119
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产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Mouse Fetal Liver
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亚型
- IgG2b kappa
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应用备注
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For IHC, we recommend the use of purified TER-119 mAb in our special formulation for immunohistochemistry.
Caution: Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format for in vitro and in vivo use. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4° C.
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: "The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage." in: British journal of haematology, Vol. 109, Issue 2, pp. 280-7, (2000) (PubMed).
: "A developmental switch in thymic lymphocyte maturation potential occurs at the level of hematopoietic stem cells." in: Cell, Vol. 62, Issue 5, pp. 863-74, (1990) (PubMed).
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- TER-119/Erythroid Cells
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背景
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The TER-119 antibody reacts with a 52 kDa molecule associated with glycophorin A on cells of the erythroid lineage in embryonic yolk sac, fetal liver, newborn liver, adult bone marrow, adult peripheral blood, and adult lymphoid organs. The TER-119 antigen is expressed on erythroid cells from pro-erythroblast through mature erythrocyte stages, but not on cells with BFU-E or CFU-E activities. The TER-119 epitope is not detected on hematopoietic stem cells, lymphoid cells, myeloid cells, or erythroleukemia lines. The TER-119 mAb is a component of the lineage cocktail used in studies of hematopoietic progenitors to detect, or deplete cells committed to the hematopoietic lineages. This antibody is routinely tested by flow cytometric analysis.
Synonyms: Ly-76
抗原
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