MHC, Class I 抗体
(3 references)Quick Overview for MHC, Class I 抗体 (ABIN967374)
抗原
See all MHC, Class I 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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品牌
- BD Pharmingen™
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产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- BALB/c mouse cells
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亚型
- IgG2a kappa
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anti-MHC, Class I antibody
适用: 人, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-MHC, Class I antibody适用: 绵羊, Cat FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal VPM19 unconjugated
anti-MHC, Class I antibody适用: 小鼠 IHC, IP, IA 宿主: 兔 Chimeric R1-21-2 unconjugated Recombinant Antibody
anti-MHC, Class I (AA 81-180) antibody适用: 人 WB, ELISA, IHC (fro), IF (cc), IF (p), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-MHC, Class I antibody适用: 小鼠 IP, IF, IA 宿主: 兔 Chimeric R1-9-6 unconjugated Recombinant Antibody
anti-MHC, Class I antibody (PE)适用: 小鸡 FACS, IHC 宿主: 小鼠 Monoclonal F21-2 PE
anti-MHC, Class I antibody适用: 小鸡 FACS, WB, IHC, IP 宿主: 小鼠 Monoclonal F21-2 unconjugated
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应用备注
- For immunohistochemical staining (IHC) of acetone-fixed frozen sections, we recommend the use of biotinylated SF1-1.1 mAb.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4° C.
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: "Alloreactive monoclonal antibodies select Kd molecules with different peptide profiles." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 6, pp. 2455-61, (1996) (PubMed).
: "Cross-linking of the TCR-CD3 complex with CD4, CD8 or LFA-1 induces an anti-apoptotic signal in thymocytes: the signal is canceled by FK506." in: International immunology, Vol. 7, Issue 9, pp. 1387-96, (1996) (PubMed).
: "Differential role of conserved and polymorphic residues of the binding groove of MHC class I molecules in the selection of peptides." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 7, pp. 3569-75, (1993) (PubMed).
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: "Alloreactive monoclonal antibodies select Kd molecules with different peptide profiles." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 6, pp. 2455-61, (1996) (PubMed).
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- MHC, Class I
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别名
- H-2K d
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背景
- The SF1-1.1 antibody reacts with the alpha3 domain of the H-2K[d] MHC class I alloantigen. Reactivity with other haplotypes (e.g, b, j, k, p, q, s, v) has not been observed. It has been reported that plate-bound SF1-1.1 mAb moderately enhances the apoptotic response of thymocytes to plate-bound 145-2C11 mAb (anti-mouse CD3e, ABIN967568 and ABIN967370). This antibody is routinely tested by flow cytometric analysis.
抗原
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