(3 references)-
- 抗原 See all MED23 抗体
- MED23 (Mediator Complex Subunit 23 (MED23))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This MED23 antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 品牌
- BD Pharmingen™
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human Sur-2 synthetic peptide
- 克隆位点
- D27-1805
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product MED23 Primary Antibody
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anti-Mediator Complex Subunit 23 (MED23) (AA 1014-1368) antibody
MED23 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 23 (MED23) antibodyMED23 适用: 人, 大鼠, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 23 (MED23) (N-Term) antibodyMED23 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 23 (MED23) (N-Term) antibodyMED23 适用: 人, 大鼠, 小鼠 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 23 (MED23) (AA 531-630) antibodyMED23 适用: 人 ELISA 宿主: 小鼠 Monoclonal 4H6 unconjugated
anti-Mediator Complex Subunit 23 (MED23) (N-Term) antibodyMED23 适用: 人, 大鼠, 小鼠, 马, 兔, Cow, 豚鼠, 猴, 小鸡 WB 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 23 (MED23) (AA 1-50) antibodyMED23 适用: 人, 大鼠, 小鼠 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 23 (MED23) (AA 897-916) antibodyMED23 适用: 人 ELISA, WB, IP 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Applications include western blot analysis (0.2-5.0 µg/ml). HeLa nuclear extracts are recommended as a positive control.
- 说明
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Related Products: ABIN967389
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "CREB-binding protein and p300: molecular integrators of hematopoietic transcription." in: Blood, Vol. 95, Issue 3, pp. 745-55, (2000) (PubMed).
: "Mammalian Srb/Mediator complex is targeted by adenovirus E1A protein." in: Nature, Vol. 399, Issue 6733, pp. 276-9, (1999) (PubMed).
: "sur-2, a novel gene, functions late in the let-60 ras-mediated signaling pathway during Caenorhabditis elegans vulval induction." in: Genes & development, Vol. 9, Issue 18, pp. 2251-65, (1995) (PubMed).
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: "CREB-binding protein and p300: molecular integrators of hematopoietic transcription." in: Blood, Vol. 95, Issue 3, pp. 745-55, (2000) (PubMed).
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- 抗原
- MED23 (Mediator Complex Subunit 23 (MED23))
- 别名
- Sur-2 (MED23 产品)
- 背景
- SUR-2 (suppressor of ras) is a gene that was originally discovered in the vulval signaling pathway of the nematode Caenorhabditis elegans. Genetic studies suggest that the product of the SUR-2 gene encodes a protein Sur-2, that acts downstream of the Raf/Map kinase pathway. The human homolog of SUR-2 (hSUR-2) was shown to interact with the E1A zinc finger domain from adenovirus. The adenovirus E1A protein can bind to a number of cellular proteins to alter cell cycle progression. E1A exerts this change in the cell cycle by interacting with the transcriptional machinery of the cell. In vitro studies indicate that Sur-2 interacts with the E1A zinc finger protein and the Srb/Mediator transcriptional multiprotein complex. These studies suggest that Sur-2 may play a role as a bridge between E1A and the Srb/Mediator multiprotein complex. However, the roles of Sur-2 in regulating the transcriptional activity of the Srb/Mediator complex and E1A remain to be fully elucidated. Sur-2 has been reported to migrate in SDS/PAGE at 150 kDa2, but our studies indicate that it migrates at approximately 140 kDa. The antibody recognizes human Sur-2. A synthetic peptide corresponding to the N-terminus region of human Sur-2 (METQLQSIFEEVVKTEVIEC) was used as immunogen.
- 分子量
- 140 kDa
- 途径
- Regulation of Lipid Metabolism by PPARalpha
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