(4 references)-
- 抗原
- SIAIOSYI-TN
- 适用
- 人
- 宿主
- 小鼠
- 克隆类型
- 单克隆
- 应用范围
- Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
- 特异性
- This antibody reacts with sialosyl-Tn antigen, [NeuAca2-6 GalNac-Ser (Thr)]. It shows no cross-reactivity with aGalNac-Ser (Thr).
- 纯化方法
- Concentrated.
- 免疫原
- Sialosyl-Tn peptide.
- 克隆位点
- B35-1
- 亚型
- IgG1 kappa
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- 应用备注
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Immunohistochemistry: 1:50-1:100
Western blotting: 1:100-1:500
ELISA 1:2000-1:5000.
Staining Procedure: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. However, prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50-1:100 in IHC. This product can be also used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory. - 说明
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Recommended Positive Control: Colon Carcinoma, Ovarian Mucinous Carcinoma
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.4 mg/ml.
- 缓冲液
- 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
-
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: "Expression of Tn, sialosyl-Tn, and T antigens in human colon cancer." in: Cancer research, Vol. 49, Issue 1, pp. 197-204, (1989) (PubMed).
: "Monoclonal antibody B72.3 reacts with a core region structure of O-linked carbohydrates." in: Tumour biology, Vol. 9, Issue 2-3, pp. 137-44, (1988) (PubMed).
: "Preparation and characterization of monoclonal antibodies directed to the tumor-associated O-linked sialosyl-2----6 alpha-N-acetylgalactosaminyl (sialosyl-Tn) epitope." in: Cancer research, Vol. 48, Issue 8, pp. 2214-20, (1988) (PubMed).
: "Studies on the mouse mammary tumor agent; survival and propagation of the agent in transplanted tumors and in hosts that grew these tumors in their tissues." in: Cancer research, Vol. 9, Issue 3, pp. 137, (1949) (PubMed).
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: "Expression of Tn, sialosyl-Tn, and T antigens in human colon cancer." in: Cancer research, Vol. 49, Issue 1, pp. 197-204, (1989) (PubMed).
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- 抗原
- SIAIOSYI-TN
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