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SIAIOSYI-TN 抗体

There are 4+ publications for this product available. The 小鼠 单克隆 anti-SIAIOSYI-TN antibody is suitable to detect SIAIOSYI-TN in samples from 人. It has been validated for IHC, ELISA 和 WB.
产品编号 ABIN967028
发货至: 中国
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Quick Overview for SIAIOSYI-TN 抗体 (ABIN967028)

抗原

SIAIOSYI-TN

适用

宿主

小鼠

克隆类型

单克隆

应用范围

Immunohistochemistry (IHC), ELISA, Western Blotting (WB)

克隆位点

B35-1
  • 特异性

    This antibody reacts with sialosyl-Tn antigen, [NeuAca2-6 GalNac-Ser (Thr)]. It shows no cross-reactivity with aGalNac-Ser (Thr).

    纯化方法

    Concentrated.

    免疫原

    Sialosyl-Tn peptide.

    亚型

    IgG1 kappa
  • 应用备注

    Immunohistochemistry: 1:50-1:100
    Western blotting: 1:100-1:500
    ELISA 1:2000-1:5000.
    Staining Procedure: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. However, prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50-1:100 in IHC. This product can be also used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory.

    说明

    Recommended Positive Control: Colon Carcinoma, Ovarian Mucinous Carcinoma

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.4 mg/ml.

    缓冲液

    20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C
  • Itzkowitz, Yuan, Montgomery, Kjeldsen, Takahashi, Bigbee, Kim: "Expression of Tn, sialosyl-Tn, and T antigens in human colon cancer." in: Cancer research, Vol. 49, Issue 1, pp. 197-204, (1989) (PubMed).

    Gold, Mattes: "Monoclonal antibody B72.3 reacts with a core region structure of O-linked carbohydrates." in: Tumour biology, Vol. 9, Issue 2-3, pp. 137-44, (1988) (PubMed).

    Kjeldsen, Clausen, Hirohashi, Ogawa, Iijima, Hakomori: "Preparation and characterization of monoclonal antibodies directed to the tumor-associated O-linked sialosyl-2----6 alpha-N-acetylgalactosaminyl (sialosyl-Tn) epitope." in: Cancer research, Vol. 48, Issue 8, pp. 2214-20, (1988) (PubMed).

    HUMMEL, LITTLE: "Studies on the mouse mammary tumor agent; survival and propagation of the agent in transplanted tumors and in hosts that grew these tumors in their tissues." in: Cancer research, Vol. 9, Issue 3, pp. 137, (1949) (PubMed).

  • 抗原

    SIAIOSYI-TN
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