GST 抗体
(1 reference)Quick Overview for GST 抗体 (ABIN966238)
抗原
See all GST 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Ni-NTA purified recombinant GST expressed in E. Coli strain M15.
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纯化方法
- Antibodies are purified by protein A affinity chromatography
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亚型
- IgG1
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anti-Glutathione S Transferase (GST) antibody
GST 适用: 标记 WB 宿主: 山羊 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibody (Biotin)GST 适用: 化学剂 WB, ELISA 宿主: 小鼠 Monoclonal vpg66 Biotin
anti-Glutathione S Transferase (GST) antibodyGST 适用: Schistosoma japonicum WB, ELISA, IP 宿主: 小鼠 Monoclonal 2F10B9 unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: Schistosoma japonicum WB, ELISA, IP, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: Schistosoma japonicum WB, ELISA, IP, DB 宿主: 小鼠 Monoclonal GST-B6 unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: 标记 WB 宿主: 山羊 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: Nicotiana tabacum WB 宿主: 兔 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: 标记 WB 宿主: 兔 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: 标记 WB, ELISA, IP, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 6G9C6 unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1E5 unconjugated
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应用备注
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Western Blot: Dilution 1: 2000- 5,000
ELISA: Proposed dilution 1: 10,000.
Determining optimal working dilutions by titration test. -
限制
- 仅限研究用
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储存条件
- -20 °C
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: "Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase." in: Gene, Vol. 67, Issue 1, pp. 31-40, (1988) (PubMed).
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: "Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase." in: Gene, Vol. 67, Issue 1, pp. 31-40, (1988) (PubMed).
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- GST (Glutathione S Transferase (GST))
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别名
- Glutathione-S-Transferase (GST)
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背景
- Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coliare in common usage, such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.
抗原
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