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GNRHR 抗体 (Extracellular Domain)

Cited in 4+ publications. This 小鼠 单克隆 anti-GNRHR antibody (Clone B470) specifically detects GNRHR in WB, ELISA, FACS 和 IHC (fro). The antibody is reactive with 人 samples.
产品编号 ABIN966217
发货至: 中国
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Quick Overview for GNRHR 抗体 (Extracellular Domain) (ABIN966217)

抗原

See all GNRHR 抗体
GNRHR (Gonadotropin-Releasing Hormone Receptor (GNRHR))

适用

  • 70
  • 18
  • 13
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  • 1

宿主

  • 43
  • 30
  • 1
小鼠

克隆类型

  • 41
  • 33
单克隆

标记

  • 37
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This GNRHR antibody is un-conjugated

应用范围

  • 29
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Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

B470
  • 抗原表位

    • 26
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    AA 1-29, Extracellular Domain

    特异性

    This antibody reacts with GnrH receptors found in the anterior pituitary. GnrH functions as a stimulator of gonadotrophs to release luteinizing hormone (LH) and follicle stimulating hormone (FSH).

    纯化方法

    Concentrated.

    免疫原

    BSA-conjugated peptide (amino acids1-29) of human GnrH receptor extracellular domain.

    亚型

    IgG1
  • 应用备注

    Immunohistochemistry: 1:50-1:100.
    ELISA: 1:1000-1:3000.
    Flow cytometry: 1:150-1:300.
    Western Blot 1:200-1:500.
    Staining Procedure: This antibody can be used on frozen tissue sections. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.

    说明

    Recommended Positive Control: Pituitary Tissue.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.3 mg/ml.

    缓冲液

    20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C
  • Grosse, Schöneberg, Schultz, Gudermann: "Inhibition of gonadotropin-releasing hormone receptor signaling by expression of a splice variant of the human receptor." in: Molecular endocrinology (Baltimore, Md.), Vol. 11, Issue 9, pp. 1305-18, (1997) (PubMed).

    Jennes, Eyigor, Janovick, Conn: "Brain gonadotropin releasing hormone receptors: localization and regulation." in: Recent progress in hormone research, Vol. 52, pp. 475-90; discussion 490-1, (1997) (PubMed).

    Fraser, Sellar, Illingworth, Eidne: "GnRH receptor mRNA expression by in-situ hybridization in the primate pituitary and ovary." in: Molecular human reproduction, Vol. 2, Issue 2, pp. 117-21, (1997) (PubMed).

    Arora, Cheng, Catt: "Mutations of the conserved DRS motif in the second intracellular loop of the gonadotropin-releasing hormone receptor affect expression, activation, and internalization." in: Molecular endocrinology (Baltimore, Md.), Vol. 11, Issue 9, pp. 1203-12, (1997) (PubMed).

  • 抗原

    GNRHR (Gonadotropin-Releasing Hormone Receptor (GNRHR))

    别名

    GnRH Receptor

    基因ID

    2798
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