Cytokeratin 19 抗体 (AA 312-335)
Quick Overview for Cytokeratin 19 抗体 (AA 312-335) (ABIN965960)
抗原
See all Cytokeratin 19 (KRT19) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 312-335
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纯化方法
- Concentrated.
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免疫原
- Keratin fraction isolated from Human Breast Cancer MCF-7 cells
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亚型
- IgG2a
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应用备注
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Immunohistochemistry: (Paraffin/Frozen): 1:50-1:100
WB: 1:100-1:500.
Staining Procedure: Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 to 1:100 with. The optimal conditions should be determined by the individual laboratory. High temperature antigen unmasking technique predigestion is strongly recommended. -
说明
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Cellular Localization: Cytoplasmic.
Recommended Positive Control: Skin, Colonic Carcinoma -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.3 mg/ml
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缓冲液
- 20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, pH 7.5
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))
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背景
- This antibody reacts with Cytokeratin 19 (40 kD) intermediate filament and with a wide variety of Simple epithelia and non-squamous stratified epithelia. It does not react with stratified squamous epithelia. However, cervical squamous cell carcinomas show positivity with this antibody.
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基因ID
- 3880
抗原
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