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Cytokeratin 19 抗体 (AA 312-335)

This anti-Cytokeratin 19 antibody is a 小鼠 单克隆 antibody detecting Cytokeratin 19 in WB, IHC (p) 和 IHC (fro). Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN965960
发货至: 中国

Quick Overview for Cytokeratin 19 抗体 (AA 312-335) (ABIN965960)

抗原

See all Cytokeratin 19 (KRT19) 抗体
Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

适用

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人, 小鼠, 大鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This Cytokeratin 19 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

B303 (A53-B-A2)
  • 抗原表位

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    AA 312-335

    纯化方法

    Concentrated.

    免疫原

    Keratin fraction isolated from Human Breast Cancer MCF-7 cells

    亚型

    IgG2a
  • 应用备注

    Immunohistochemistry: (Paraffin/Frozen): 1:50-1:100
    WB: 1:100-1:500.
    Staining Procedure: Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 to 1:100 with. The optimal conditions should be determined by the individual laboratory. High temperature antigen unmasking technique predigestion is strongly recommended.

    说明

    Cellular Localization: Cytoplasmic.
    Recommended Positive Control: Skin, Colonic Carcinoma

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.3 mg/ml

    缓冲液

    20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, pH 7.5

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C
  • 抗原

    Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

    背景

    This antibody reacts with Cytokeratin 19 (40 kD) intermediate filament and with a wide variety of Simple epithelia and non-squamous stratified epithelia. It does not react with stratified squamous epithelia. However, cervical squamous cell carcinomas show positivity with this antibody.

    基因ID

    3880
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