CD74, MHC CLASS11 INVARIANT CHAIN 抗体
适用: 人
IHC (fro), FACS, IF, WB
宿主: 小鼠
Monoclonal
B318 (LN-2)
unconjugated
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- 抗原
- CD74, MHC Class 11 invariant Chain
- 适用
- 人
- 宿主
- 小鼠
- 克隆类型
- 单克隆
- 应用范围
- Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
- 特异性
- This antibody reacts with several isoforms of the CD74 antigen, the invariant chain of HLA-DR.
- 纯化方法
- Purified.
- 免疫原
- Cell extracts from diffuse histolytic lymphoma SU-DHL-4 cells.
- 克隆位点
- B318 (LN-2)
- 亚型
- IgG1
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- 应用备注
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Immunofluorescence: 10-20 ug/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Immunoblotting: 1-5 ug/mL.
Immunohistochemistry: 10-20 ug/mL on frozen or formalin fixed paraffin embedded tissue sections using avidin-biotin system.
This product was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope. The antibody may be used at a dilution of 10-20 ug/mL in IHC. The optimal conditions should be determined by the individual laboratory. When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique (see instructions below) is strongly recommended for consistent and reproducible results This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope. The antibody may be used at a dilution of 10-20 ug/mL in IHC. The optimal conditions should be determined by the individual laboratory. When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique is strongly recommended for consistent and reproducible results - 说明
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Recommended Positive Control: Tonsil
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.4 mg/ml.
- 缓冲液
- 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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- 抗原
- CD74, MHC Class 11 invariant Chain
- 别名
- CD74,MHC Class11 Invariant Chain
- 基因ID
- 972
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