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CD56 抗体

This anti-CD56 antibody is a 小鼠 单克隆 antibody detecting CD56 in FACS 和 IHC (fro). Suitable for 人.
产品编号 ABIN7446175
发货至: 中国

Quick Overview for CD56 抗体 (ABIN7446175)

抗原

See all CD56 (NCAM1) 抗体
CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD56 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

B332(123A8)
  • 特异性

    This antibody reacts with a 135/220 kD glycosylated and sialyated protein, expressed on CD2+, CD3-, CD16+ natural killer cells and neuroectodermal cells.

    纯化方法

    Purified.

    免疫原

    Membrane preparation from small cell lung carcinoma.

    亚型

    IgG1
  • 应用备注

    Immunofluorescence: 10-20 micrograms/mL.
    Flow cytometry: 1-5 ug/10^6 cells.
    Westernblotting: 1-5 ug/mL.
    Immunohistochemistry: 4 -5 micrograms/mL on frozen and formalin fixed tissue sections using avidin-biotin system. The formalin fixed tissue requires boiling in 10 mM citrate buffer, pH 6.0 for 10-20 mins. This antibody is used for identification of NK- cells.
    This product was not quality controlled in flow cytometry.
    Staining Procedure: This antibody can be used on acetone fixed frozen cryostat sections. This antibody can be used with formalin-fixed paraffin-embedded tissue sections. These tissue require boiling in 10 mM citrate buffer, pH 6.0 for 10-20 mins. followed by cooling at RT. The antibody may be used at a dilution of 2-4 micrograms/mL in IHC.

    说明

    Recommended Positive Control: Human retinoblastoma or neuroblastoma, small Granulocytes Lung Carcinoma or wilms tumors.

    限制

    仅限研究用
  • 浓度

    0.2 mg/ml.

    缓冲液

    10 mM Sodium phosphate, 150 mM Sodium Chloride, 0.2% BSA, pH 7.4

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C
  • 抗原

    CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

    别名

    CD56,Natural Killer Cell

    分子量

    125, 145, 180 kDa.

    基因ID

    4684
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