CD56 抗体
Quick Overview for CD56 抗体 (ABIN7446175)
抗原
See all CD56 (NCAM1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody reacts with a 135/220 kD glycosylated and sialyated protein, expressed on CD2+, CD3-, CD16+ natural killer cells and neuroectodermal cells.
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纯化方法
- Purified.
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免疫原
- Membrane preparation from small cell lung carcinoma.
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亚型
- IgG1
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应用备注
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Immunofluorescence: 10-20 micrograms/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Westernblotting: 1-5 ug/mL.
Immunohistochemistry: 4 -5 micrograms/mL on frozen and formalin fixed tissue sections using avidin-biotin system. The formalin fixed tissue requires boiling in 10 mM citrate buffer, pH 6.0 for 10-20 mins. This antibody is used for identification of NK- cells.
This product was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on acetone fixed frozen cryostat sections. This antibody can be used with formalin-fixed paraffin-embedded tissue sections. These tissue require boiling in 10 mM citrate buffer, pH 6.0 for 10-20 mins. followed by cooling at RT. The antibody may be used at a dilution of 2-4 micrograms/mL in IHC. -
说明
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Recommended Positive Control: Human retinoblastoma or neuroblastoma, small Granulocytes Lung Carcinoma or wilms tumors.
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限制
- 仅限研究用
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浓度
- 0.2 mg/ml.
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缓冲液
- 10 mM Sodium phosphate, 150 mM Sodium Chloride, 0.2% BSA, pH 7.4
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))
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别名
- CD56,Natural Killer Cell
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分子量
- 125, 145, 180 kDa.
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基因ID
- 4684
抗原
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