CD43A 抗体
适用: 人
IHC (fro), FACS, IF
宿主: 小鼠
Monoclonal
B316 (DFT1)
unconjugated
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- 抗原
- CD43A
- 适用
- 人
- 宿主
- 小鼠
- 克隆类型
- 单克隆
- 应用范围
- Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF)
- 特异性
- This antibody reacts with a 95-115 kD protein on T-cells and thymocytes and a 115-135 kD molecules on neutrophils and platelets. These proteins are o-glycosylated and are mucin-like molecules with different antigenic epitopes.
- 纯化方法
- Purified.
- 免疫原
- The myeloblast cell line KG1.
- 克隆位点
- B316 (DFT1)
- 亚型
- IgG1 kappa
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- 应用备注
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Immunofluorescence: 10-20 ug/mL.
Flow cytometry: 1 ug/10^6 cells.
Immunoblotting: 1-5 ug/mL.
Immunohistochemistry: 5-10 ug/mL on frozen and formalin fixed sections using avidin-biotin system. This antibody was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope. The antibody may be used at a dilution of 5-10 ug/mL in IHC. High temperature antigenic unmasking technique is recommended to enhance staining on formalin fixed tissue sections. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory. - 说明
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Recommended Positive Control: Lymphoid Tissue sections
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.4 mg/ml.
- 缓冲液
- 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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- 抗原
- CD43A
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