CD20 (B Cells) (Extracellular Domain) 抗体
Cited in 2+ publications. This anti-CD20 (B Cells) antibody is a 小鼠 单克隆 antibody detecting CD20 (B Cells) in FACS, IF 和 IHC (fro). Suitable for 人.
(2 references)
产品编号 ABIN965777
发货至:
中国
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Quick Overview for CD20 (B Cells) (Extracellular Domain) 抗体 (ABIN965777)
抗原
CD20 (B Cells)
适用
人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
克隆位点
B342 (B9E9)
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抗原表位
- Extracellular Domain
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特异性
- This antibody reacts with an antigen present on most of the B-cells. They react with a 33 kD polypeptide and a minor component of a 30 kD polypeptide present in B-cells.
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纯化方法
- Purified.
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免疫原
- Lymphoblastoid cell line DAUDI.
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亚型
- IgG2a
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anti-CD20 (B Cells) antibody
适用: 人 FACS, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD20 (B Cells) antibody适用: 人 FACS, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunofluorescence: 5-20 ug/mL
Flow cytometry: 1-5 ug/10^6 cells
Western blotting: 1-5 ug/mL.
Immunohistochemistry: 5-20 mg/mL on frozen sections using avidin-biotin system. This antibody was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on frozen cryostat sections. -
说明
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Cellular Localization: Cell membrane.
Recommended Positive Control: Tonsil and Lymph Node -
限制
- 仅限研究用
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浓度
- 0.2 mg/ml.
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缓冲液
- 50 mM Sodium phosphate, 150 mM Sodium Chloride, BSA 0.2%, pH 8.0
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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: "Expression of calcium-permeable cation channel CD20 accelerates progression through the G1 phase in Balb/c 3T3 cells." in: The Journal of biological chemistry, Vol. 270, Issue 22, pp. 13099-104, (1995) (PubMed).
: "Transfection of the CD20 cell surface molecule into ectopic cell types generates a Ca2+ conductance found constitutively in B lymphocytes." in: The Journal of cell biology, Vol. 121, Issue 5, pp. 1121-32, (1993) (PubMed).
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: "Expression of calcium-permeable cation channel CD20 accelerates progression through the G1 phase in Balb/c 3T3 cells." in: The Journal of biological chemistry, Vol. 270, Issue 22, pp. 13099-104, (1995) (PubMed).
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- CD20 (B Cells)
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分子量
- 33-37 kDa
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基因ID
- 931
抗原
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