TNNC1 抗体
Quick Overview for TNNC1 抗体 (ABIN965749)
抗原
See all TNNC1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody is specific for human cardiac troponin subunit I, a 21 kD protein responsible for inhibiting actomyosin Mg2+-ATPase activity. This antibody has less than 0.1% reactivity with human skeletal TNI as measured by ELISA.
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产品特性
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High temp. antigen demasking for FFPE tissue sections. Deparaffinize and rehydrate tissue sections.
10mM citrate buffer: pH 6.0. Boil 10-20 mins, followed by cooling 10-20 mins
OR Process tissue sections in Pressure cooker for 5 mins in citrate buffer followed by cooling 10-20 mins to RT. Wash tissue sections with buffer (PBS or Automation buffer,) -
纯化方法
- Concentrated.
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免疫原
- Purified human cardiac troponin I.
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亚型
- IgG1 kappa
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anti-Cardiac Troponin C (TNNC1) (AA 131-210) antibody
TNNC1 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IF (p), IHC (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cardiac Troponin C (TNNC1) (AA 1-161) antibodyTNNC1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cardiac Troponin C (TNNC1) (AA 1-160) antibodyTNNC1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cardiac Troponin C (TNNC1) (AA 11-149) antibodyTNNC1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Cardiac Troponin C (TNNC1) (AA 1-161) antibodyTNNC1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1F8-A9 unconjugated
anti-Cardiac Troponin C (TNNC1) antibodyTNNC1 适用: 人 WB, IHC, ELISA, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-Cardiac Troponin C (TNNC1) (N-Term) antibodyTNNC1 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cardiac Troponin C (TNNC1) (pThr143) antibodyTNNC1 适用: 人, 小鼠, 大鼠 IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cardiac Troponin C (TNNC1) (AA 1-161) antibodyTNNC1 适用: 人 WB 宿主: 小鼠 Monoclonal C5 unconjugated
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应用备注
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Immunohistochemistry:1:25. For formalin-fixed paraffin-embedded (FFPE) tissue sections antigen demasker (10 mM citrate buffer pH 6.0 ) is required. Boil the tissue 10-20 mins in this buffer, followed by cooling to RT.
Western blotting: 1:50-1:100.
ELISA: 1:500-1:1000
Immunoprecipitation.
Staining Procedure: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:25-1:50 in IHC. When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique is strongly recommended for consistent and reproducible results. This product can also be used on frozen tissue sections. The optimal conditions should be determined by the individual laboratory. -
说明
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Cellular Localization: cytoplasmic staining of cardiac muscle cells.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.4 mg/ml.
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缓冲液
- 20 mM tris-borate, 150 mM Sodium Chloride, BMC-6 carrier polysaccharides, pH 7.5
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- TNNC1 (Cardiac Troponin C (TNNC1))
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别名
- Cardiac Troponin-1
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分子量
- 21 kDa
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基因ID
- 7134
抗原
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