我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

SAE1 抗体 (Internal Region)

Name: SAE1 抗体 (Internal Region)
SKU: ABIN964925
Availability: OutOfStock

SAE1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

Western blot using Rabbit anti-SAE1 antibody shows detection of SAE1. (SAE1 抗体 (Internal Region))

1 image

产品编号 ABIN964925

Supplier Product No.: 600-401-c51s

发货至: 中国

Datasheet as PDF

抗原 See all SAE1 抗体

SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))
抗原表位
15

8

6

6

5

4

2

2

1

1

1

1

1

1

1
Internal Region
适用
58

40

16

5

5

4

4

4

3

3

2

1

1
人
宿主
65

7

1
兔
Compatible Secondaries
克隆类型
59

14
多克隆
标记
40

4

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
This SAE1 antibody is un-conjugated
应用范围
56

24

17

15

14

13

13

7

4

3

2

1

1

1

1

1

1
Western Blotting (WB), ELISA

Supplier Product No.

600-401-c51s

Supplier

Rockland

原理

SAE1 Antibody

交叉反应（详细）

This purified antibody is directed against human SUMO Activating Enzyme E1 protein.

产品特性

Synonyms: rabbit anti-SAE1 antibody, rabbit anti-SUMO activating enzyme subunit 1 antibody, Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, SUA1, SAE-1

纯化方法

The product was purified from monospecific antiserum by affinity chromatography.

过滤

Sterile filtered

免疫原

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human SUMO Activating Enzyme E1 protein.
Immunogen Type: Conjugated Peptide

亚型

IgG

Top Product

Discover our top product SAE1 Primary Antibody
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-346) antibody
SAE1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1G4-1G5 unconjugated

anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) antibody
KO Validated SAE1 适用: 人 WB, IF, IHC (p), ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (Internal Region) antibody
SAE1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-346) antibody
SAE1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 300-329), (C-Term) antibody
SAE1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB2887-RB2888 unconjugated

anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-346) antibody
SAE1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) antibody
SAE1 适用: 人 WB, ELISA, IF 宿主: 兔 Monoclonal 9D8 unconjugated Recombinant Antibody

anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) antibody
SAE1 适用: 人 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 1G8 unconjugated

anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) antibody
SAE1 适用: 人 ICC, DB 宿主: 小鼠 Monoclonal 2946C4a unconjugated

anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (Center) antibody
SAE1 适用: 人, 猴 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated

应用备注

Application Note: This purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~37 kDa in size corresponding to SAE1 protein by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 2 μg/mL
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized

限制

仅限研究用
状态

Liquid

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
抗原

SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

别名

SAE1 (SAE1 产品)

背景

Background: SUMO E1 activating enzyme (also called Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, and SUA1) with SAE2 (also known as UBA2) forms a heterodimeric (SAE1/SAE2) enzyme that activates the ubiquitin-like SUMO proteins (SUMO stands for Small Ubiquitin-like MOdifier.) The SAE1 (SUMO Activating Enzyme 1) subunit resembles the N-terminal half of yeast UBA1, the SAE2 (also called Uba2) subunit corresponds to the C-terminal part of yeast UBA1 and contains the active site cysteine. In the SUMO activation step, SAE1/SAE2 uses ATP to adenylate the C-terminal glycine of SUMO-1 (the first of the three different mammalian SUMO proteins) then forms a high-energy thioester bond between the C-terminal glycine and the active site cysteine in SAE2 (Uba2). In the conjugation step, the SUMO moiety is transferred from SAE1/SAE2 to the active site cysteine (Cys 93) of the SUMO conjugating enzyme (SUMO E2, Ubc9) forming a SUMO-E2 thioester complex.

基因ID

10055, 42559897

UniProt

Q9UBE0