CCL4 抗体
Quick Overview for CCL4 抗体 (ABIN964760)
抗原
See all CCL4 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 209-401-b97
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Supplier
- Rockland
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原理
- MIP-1 beta Antibody
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产品特性
- Synonyms: rabbit anti-MIP-1 beta Antibody, rabbit anti-MIP-1b Antibody, CCL4, C-C motif chemokine 4, Small-inducible cytokine A4, Macrophage inflammatory protein 1-beta, MIP-1-beta, ACT-2, T-cell activation protein 2, Protein H400, Lymphocyte activation gene 1 protein, LAG-1, HC21, G-26 T-lymphocyte-secreted protein, MIP-1ß
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纯化方法
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56 °C for 30 minutes.
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免疫原
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Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP-1ß protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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anti-Chemokine (C-C Motif) Ligand 4 (CCL4) (AA 24-92) antibody
CCL4 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 4 (CCL4) (AA 24-92) antibodyCCL4 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 7C9E4 unconjugated
anti-Chemokine (C-C Motif) Ligand 4 (CCL4) (AA 24-92) antibodyCCL4 适用: 人 ELISA 宿主: 小鼠 Monoclonal 8F1C2 unconjugated
anti-Chemokine (C-C Motif) Ligand 4 (CCL4) (AA 26-92) antibodyCCL4 适用: 人, 小鼠, 兔 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 4 (CCL4) (Internal Region) antibodyCCL4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 4 (CCL4) antibodyCCL4 适用: 人 WB, ELISA, IHC (p), Func 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 4 (CCL4) (C-Term) antibodyCCL4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 4 (CCL4) (AA 24-92) antibodyCCL4 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 4 (CCL4) (AA 22-48) antibodyCCL4 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 4 (CCL4) (AA 24-92) antibodyCCL4 适用: 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This purified antibody has been tested in western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/μl by the LAL method. By western blot a band approximately 15 kDa in size corresponding to native human MIP-1ß protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:1,000
ELISA Dilution: 1:10,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: None -
储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))
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别名
- CCL4
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背景
- Background: MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a. The predicted protein products of these cDNAs represent variants that are between 94 % - 98 % identical and these proteins are all approximately 75 % homologous to murine MIP1 beta. MIP1 beta also shares approximately 70 % amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated.
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基因ID
- 6351
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NCBI登录号
- NP_002975
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UniProt
- P13236
抗原
-
