IL-2 抗体
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- 抗原 See all IL-2 (IL2) 抗体
- IL-2 (IL2) (Interleukin 2 (IL2))
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适用
- Cat
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This IL-2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- Supplier Product No.
- 202-401-b95
- Supplier
- Rockland
- 原理
- IL-2 Antibody
- 交叉反应 (详细)
- This antibody is specific for cat IL-2 protein.
- 产品特性
- Synonyms: rabbit anti-Interleukin-2, rabbit anti-IL-2 antibody, IL2, T-cell growth factor, TCGF, Aldesleukin, Lymphokine
- 纯化方法
- This product was protein A purified from monospecific antiserum by chromatography.
- 免疫原
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Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast corresponding to 134 amino acids of mature cat IL-2.
Immunogen Type: Recombinant Protein
- 亚型
- IgG
- Top Product
- Discover our top product IL2 Primary Antibody
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- 应用备注
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Application Note: This protein A purified IL-2 antibody has been tested by ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15.4 kDa in size corresponding to feline IL-2 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500-1:2,000
ELISA Dilution: 1:20,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IL-2 (IL2) (Interleukin 2 (IL2))
- 别名
- IL2 (IL2 产品)
- 背景
- Background: IL-2 is a member of a family of cytokines, which also includes IL-4, IL-7, IL-9, IL-15 and IL-21. All members of this family signal through a receptor complex that includes the common gamma chain. IL-2 signals through a receptor complex consisting of IL-2 specific IL-2 receptor alpha (CD25), IL-2 receptor beta (CD122) and the common gamma chain. The main function of IL-2 is to promote the development of T regulatory cells. IL-2 is produced by several cell types including, B cells, gamma-delta T cells, CD4+ T cells, CD8+ T cells, and eosinophils. It has been found to be similar to IL-15 in terms of function. Both IL-2 and IL-15 are able to facilitate production of immunoglobulins made by B cells and induce the differentiation and proliferation of natural killer cells. The primary differences between IL-2 and IL-15 are found in adaptive immune responses: IL-2 participates in maintenance of T-Regs and reduces self-reactive T cells while IL-15 is necessary for maintaining highly specific T cell responses by supporting survival of CD8 memory T cells.
- 基因ID
- 751114
- NCBI登录号
- NP_001036802
- UniProt
- Q07885
- 途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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