FIL1d 抗体
Quick Overview for FIL1d 抗体 (ABIN964734)
抗原
See all FIL1d 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 201-401-b77
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Supplier
- Rockland
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原理
- IL-1F5 Antibody
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交叉反应 (详细)
- This antibody is specific for bovine IL-1F5 protein.
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产品特性
- Synonyms: rabbit anti-IL-1F5 antibody, rabbit anti-Interleukin 1 family, member 5 (Delta)
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纯化方法
- This product was Protein-A purified from monospecific antiserum by chromatography.
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免疫原
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Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 155 amino acids of the mature bovine IL-1F5 protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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应用备注
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Application Note: This protein-A purified Interleukin-1F5 antibody has been tested by ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 17.1 kDa in size corresponding to bovine IL-1F5 protein by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1 μg/mL
ELISA Dilution: 8,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 µg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- FIL1d (Interleukin 1 delta (FIL1d))
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别名
- IL1F5
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背景
- Background: Interleukin-1F5 is a recently discovered cytokine which was shown to specifically inhibit the activation of NF-kappaB induced by interleukin 1 family, member 6 (IL1F6) in humans. There is very little published regarding the bovine IL1F5 protein. In the human, this gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Expression of the gene appears to be restricted to various epithelial cell populations. Costelloe et al (2008) have identified SIGIRR [single immunoglobulin domain-containing IL1R-related protein] as a receptor for human IL1F5. Anti-IL-1F5 antibody is ideal for investigators involved in Signal Transduction and Immunology research.
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基因ID
- 518514, 115494990
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UniProt
- Q0VC52
抗原
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