Surface Lipoprotein P27 (BB_A60) 抗体
Rockland 200-401-c30
BB_A60
适用: Borrelia burgdorferi
ELISA, WB
宿主: 兔
Polyclonal
unconjugated
1 image
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- 抗原
- Surface Lipoprotein P27 (BB_A60)
- 适用
- Borrelia burgdorferi
- 宿主
- 兔
- 克隆类型
- 多克隆
- 应用范围
- ELISA, Western Blotting (WB)
- Supplier Product No.
- 200-401-c30
- Supplier
- Rockland
- 原理
- Surface Lipoprotein p27 Antibody
- 交叉反应 （详细）
- It is directed against, and shows specific reactivity for, Borrelia burgdorferi p27 protein.
- 产品特性
- Synonyms: rabbit anti-Surface Lipoprotein p27 Antibody, BBA060 protein, Borrelia burgdorferi p27
- 纯化方法
- This antibody was purified from monospecific antiserum by protein-A purified immunoaffinity chromatography, and cross-adsorbed against MBP.
- 免疫原
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Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi Surface Lipoprotein p27 protein.
Immunogen Type: Recombinant Protein
- 亚型
- IgG
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- 应用备注
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Application Note: Anti-Surface Lipoprotein p27 antibody has been tested in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~30.9 kDa in size corresponding to p27 by Western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1,000
ELISA Dilution: 1:1,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Surface Lipoprotein P27 (BB_A60)
- 别名
- BB_A60
- 背景
- Background: Surface Lipoprotein p27 of Borrelia burgdorferi is a surface-exposed lipoprotein that has been shown (by Western blot and Northern blot) to be expressed in the European B. burgdorferi strain B29, but not in the American strain B31. Cell envelope proteins of bacterial pathogens play important roles in the host-parasite interactions that occur during infection, including cell adherence, cell invasion, and immune cell activation or evasion. p27 is a basic protein of 248 amino acids with a typical prokaryotic leader sequence of 17 amino acid residues at the N-terminus of the proposed translation product. The p27 gene is located on a linear plasmid of a size of approximately 55 kb. Borrelia spirochetes are unique among diderm bacteria in their abundance of surface-displayed lipoproteins, some of which play important roles in the pathogenesis of Lyme disease and relapsing fever. There is evidence that Borrelia lipoproteins are specifically targeted to the bacterial surface, but that they can be retained in the periplasm by sequence-specific signals.
- 基因ID
- 1194336
- NCBI登录号
- WP_010890395
- UniProt
- O50951
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