Outer Surface Protein C (B. Burgdorferi) (OspC) 抗体
This anti- antibody is a 兔 多克隆 antibody detecting in ELISA 和 WB. Suitable for Borrelia burgdorferi. This Primary Antibody has been cited in 4+ publications.
1 image
(4 references)
Quick Overview for Outer Surface Protein C (B. Burgdorferi) (OspC) 抗体 (ABIN964717)
抗原
Outer Surface Protein C (B. Burgdorferi) (OspC)
适用
Borrelia burgdorferi
宿主
兔
克隆类型
多克隆
标记
非结合性
应用范围
ELISA, Western Blotting (WB)
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Supplier Product No.
- 200-401-c11
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Supplier
- Rockland
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原理
- OspC Antibody
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交叉反应 （详细）
- This antibody is specific for Borrelia burgdorferi OspC protein.
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产品特性
- Synonyms: rabbit anti-OspC Antibody, Borrelia burgdorferi OspC, PC, Outer Surface Protein C, BB_B19
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纯化方法
- This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography.
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免疫原
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Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi OspC protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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应用备注
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Application Note: This protein-A purified antibody has been tested for use in Western blotting and in ELISA. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 20.7 kDa in size corresponding to Borrelia burgdorferi OspC protein by Western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Borrelia burgdorferi Outer Membrane Vesicles Contain Antigenic Proteins, but Do Not Induce Cell Death in Human Cells." in: Microorganisms, Vol. 10, Issue 2, (2022) (PubMed).
: "Paper-based multiplexed vertical flow assay for point-of-care testing." in: Lab on a chip, Vol. 19, Issue 6, pp. 1027-1034, (2019) (PubMed).
: "Borrelia burgdorferi outer surface protein C (OspC) binds complement component C4b and confers bloodstream survival." in: Cellular microbiology, Vol. 19, Issue 12, (2018) (PubMed).
: "A short-term Borrelia burgdorferi infection model identifies tissue tropisms and bloodstream survival conferred by adhesion proteins." in: Infection and immunity, Vol. 83, Issue 8, pp. 3184-94, (2015) (PubMed).
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: "Borrelia burgdorferi Outer Membrane Vesicles Contain Antigenic Proteins, but Do Not Induce Cell Death in Human Cells." in: Microorganisms, Vol. 10, Issue 2, (2022) (PubMed).
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- Outer Surface Protein C (B. Burgdorferi) (OspC)
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别名
- ospC
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物质类
- Bacteria Protein
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背景
- Background: Outer Surface Protein C, or OspC, is a 20.7 kDa immunogenic protein on the outer surface of the spirochete Borrelia burgdorferi. Its function is not known, but it is located with lipid-anchoring sites on the outer cell membrane.
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基因ID
- 1194415
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NCBI登录号
- WP_010890595
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UniProt
- Q07337
抗原
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