LYZ 抗体 (AA 1-129)
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- 抗原 See all LYZ 抗体
- LYZ (Lysozyme (LYZ))
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抗原表位
- AA 1-129
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适用
- 小鸡
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This LYZ antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Dot Blot (DB)
- Supplier Product No.
- 200-401-072
- Supplier
- Rockland
- 原理
- Lysozyme Antibody
- 交叉反应 (详细)
- This purified antibody is directed against lysozyme from hen egg white protein.
- 产品特性
- Synonyms: rabbit anti-Lysozyme Antibody, 1 4 beta n acetylmuramidase c antibody, EC 3.2.1.17 antibody, LYZ antibody, Lysozyme C antibody, Lysozyme C precursor antibody, LZM antibody, Hen Egg White
- 纯化方法
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process, which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length protein corresponding to amino acids 1-129 of Hen Egg White Lysozyme.
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product LYZ Primary Antibody
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- 应用备注
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Immunohistochemistry Dilution: 1:500 - 1:2,000
Application Note: Anti-Lysozyme Hen Egg White purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~14 kDa in size corresponding to lysozyme by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:2,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:25,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/ml
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- LYZ (Lysozyme (LYZ))
- 别名
- LYZ (LYZ 产品)
- 背景
- Background: Lysozyme is a relatively small (129 AA) secretory enzyme that catalyzes the hydrolysis of β-1,4 glucosidic linkages between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) comprising the cell walls of bacteria and to a lesser degree chitin oligomers. Lysozyme is common in animals and plants. In birds, lysozyme is also an exceptionally abundant protein in egg whites. Its biological function in fowl eggs is unclear. Hen egg white lysozyme ''c'' is the most commonly studied form and source of the enzyme. Lysozyme from domestic goose is designated lysozyme ''g''. Lysozyme is also found in vertebrates, including human, mostly in secretions and certain tissues, such as saliva, tears, milk, cervical mucus, leucocytes, kidneys and urine, where it probably serves as an anti-bacterial agent by digesting and weakening the rigid bacterial cell wall, thereby rendering the bacteria susceptible to osmotic lysis. Plant lysozyme is found in ficus and papaya latex, and is chemically distinct from the egg white enzyme.
- 基因ID
- 396218
- NCBI登录号
- NP_990612
- UniProt
- P00698
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