DYKDDDDK Tag 抗体 (Agarose Beads)
Quick Overview for DYKDDDDK Tag 抗体 (Agarose Beads) (ABIN964701)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-350-383
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Supplier
- Rockland
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原理
- DYKDDDDK Tag (Anti-FLAG®) Antibody Agarose Conjugated
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序列
- DYKDDDDK
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交叉反应 （详细）
- This product is intended for purification of proteins containing the FLAG® epitope tag sequence.
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产品特性
- Synonyms: mouse anti-FLAG® tag agarose gel, mouse anti-DYKDDDDK agarose gel, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, D-Y-K-D-D-D-D-K, DDK, FLAG, FLAG antibody, anti-Flag, anti-DDK, DDK antibody
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纯化方法
- DYKDDDDK Tag (Anti-FLAG®) Antibody Agarose Conjugated is a purified mouse IgG2a monoclonal antibody coupled to activated agarose.
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免疫原
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Immunogen: DYKDDDDK Tag (Anti-FLAG®) Antibody Agarose Conjugated was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG® epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH.
Immunogen Type: Conjugated Peptide
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亚型
- IgG2a kappa
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anti-DYKDDDDK Tag antibody
WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal FG4R unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IP 宿主: 小鼠 Monoclonal 5A8E5 unconjugated
anti-DYKDDDDK Tag antibody适用: 标记 WB, ELISA, IP, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 2F11G1 unconjugated
anti-DYKDDDDK Tag antibodyWB, IP, IF, IC 宿主: 小鼠 Monoclonal unconjugated
anti-DYKDDDDK Tag antibody适用: 人 WB 宿主: 兔 Polyclonal RB0493 unconjugated
anti-DYKDDDDK Tag antibody (HRP)WB, ELISA, IF 宿主: 小鼠 Monoclonal 5A8E5 HRP
anti-DYKDDDDK Tag antibodyWB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 29E4-G7 unconjugated
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应用备注
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Application Note: DYKDDDDK Tag (Anti-FLAG®) Antibody Agarose Conjugated has been tested by IP and western blot and is optimally suited for immunoprecipitation and purification of FLAG® tagged fusion proteins.DYKDDDDK Tag (Anti-FLAG®) Antibody Agarose Conjugated recognizes the FLAG® epitope tag fused to either the amino- or carboxy-terminal ends or an internal location of targeted fusion proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. Use D-Y-K-D-D-D-D-K peptide (p/n 000-000-383) for competitive elution to recover fusion protein (see protocol). Anti-FLAG® is a registered trademark of Sigma-Aldrich. Refer to the protocol for complete instructions for use including preferred buffers for elution. Do not use buffers that may denature the anti-DYKDDDDK antibody.
Immunoprecipitation Dilution: 10 μL resin binds >1 μg FLAG® fusion protein
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限制
- 仅限研究用
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4°C prior to opening.
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有效期
- 12 months
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- DYKDDDDK Tag
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别名
- FLAG
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物质类
- Tag
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背景
- Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Anti-epitope tag antibodies serve as universal detection reagents for any tag containing protein produced by recombinant means. Epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.
抗原
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