-
- 抗原 See all NEDD1 抗体
- NEDD1 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1))
-
适用
- 人
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This NEDD1 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), ELISA
- Supplier Product No.
- 109-401-c38
- Supplier
- Rockland
- 原理
- NEDD1 Antibody
- 交叉反应 （详细）
- This antibody reacts with endogenous Nedd1 protein.
- 产品特性
- Synonyms: GCP-WD, Neural precursor cell expressed developmentally down-regulated protein 1, rabbit anti-NEDD1 Antibody
- 纯化方法
- This product was adsorbed against GST from monospecific antiserum by immunoaffinity chromatography.
- 过滤
- Sterile filtered
- 免疫原
-
Immunogen: Anti-NEDD1 was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the 343-667 region of human Nedd1.
Immunogen Type: Recombinant Protein
- Top Product
- Discover our top product NEDD1 Primary Antibody
-
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) (AA 561-660) antibody
NEDD1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 7D10 unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) (AA 411-660) antibodyNEDD1 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) (AA 411-660) antibodyNEDD1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) antibodyNEDD1 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) (Internal Region) antibodyVerified NEDD1 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) (N-Term) antibodyNEDD1 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 兔, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1) (N-Term) antibodyNEDD1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
Application Note: This antiserum has been tested for use in ELISA and western blotting using a recombinant truncated Nedd1 protein. Specific conditions for reactivity and detection of Nedd1 should be optimized by the end user. Expect a band approximately ~73 kDa in size corresponding to Nedd1 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:10,000
ELISA Dilution: 1:100,000
Other: User Optimized
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 40 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
- 抗原
- NEDD1 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1))
- 别名
- NEDD1 (NEDD1 产品)
- 背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Microtubules are polymers of tubulin, which exist as heterodimers of alpha-tubulin and beta-tubulin. NEDD1 (neural precursor expressed, developmentally down-regulated protein1, also called GCP-WD) is a centrosomal protein that in mammals associates with the gamma-tubulin ring complex. Gamma-TuRC is critical for initiation, or nucleation, of the microtubule assembly. In association with this complex, NEDD1 plays an important role in targeting the gamma-TuRC complex to the site of microtubule nucleation and to the mitotic spindle. These events are essential for proper bipolar spindle formation and mitotic progression. Given the casual link between improper spindle function and tumorigenesis, characterization of Nedd1 function will be important to better understand various mechanisms underlying mitotic regulation, chromosome segregation, and cancer development.
- 基因ID
- 121441
- NCBI登录号
- NP_001128647
- 途径
- M Phase
-
You are here:
