Tetanus Toxin C-Fragment (TTC) 抗体
适用: Clostridium tetani
ELISA, WB
宿主: 兔
Polyclonal
unconjugated
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- 抗原
- Tetanus Toxin C-Fragment (TTC)
- 适用
- Clostridium tetani
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
- 应用范围
- ELISA, Western Blotting (WB)
- Supplier Product No.
- 100-401-894
- Supplier
- Rockland
- 原理
- Tetanus Toxin Fragment C Antibody
- 交叉反应 （详细）
- This antibody is directed against the C fragment of Tetanus toxin derived from Clostridium tetani.
- 产品特性
- Synonyms: rabbit anti-Tetanus Toxin Fragment C Antibody , TTfC, tentoxylysin, tetX
- 纯化方法
- The product was prepared from monospecific antiserum by delipidation and defibrination.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a polyhistidine-tagged TTFC fusion protein expressed in E. coli corresponding to full length TTFC.
Immunogen Type: Recombinant Protein
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- 应用备注
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Application Note: This antibody has been tested for use in ELISA and western blot. For western blots expect a band of approximately 52 kDa in size corresponding to full-length TTFC. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:1,000 - 1:40,000
ELISA Dilution: 1:50,000 - 1:250,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 63 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Tetanus Toxin C-Fragment (TTC)
- 背景
- Background: Tetanus toxin C-fragment (TTc) is a non-toxic 52 kD polypeptide generated by papain cleavage of tetanus toxin, corresponding to the C-terminal 451 amino acids. The illness known as Tetanus is caused by a neurotoxin produced by the anaerobic bacterium Clostridium tetani. C. tetani is commonly found in soil, feces, and on many objects lying on the ground, such as rusty metal, hence puncture wounds are a common cause of tetanus. It acts upon the presynaptic membranes of both central and peripheral nervous systems to block the release of neurotransmitters. In its toxic form tetanus neurotoxin is a 150 kDa protein, consisting of two major components: a light chain and heavy chain. The light chain contains the enzymatic portion of the toxin and is responsible for its toxic effects. The heavy chain binds to the neuron and aids delivery of the light chain to the neuron interior. A single disulfide bond links light and heavy chains.
- 基因ID
- 1061100
- NCBI登录号
- NP_783831
- UniProt
- P04958
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