ZNF148 抗体
(2 references)
Quick Overview for ZNF148 抗体 (ABIN964672)
抗原
See all ZNF148 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 100-401-685
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Supplier
- Rockland
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原理
- ZBP-89 Antibody
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交叉反应 （详细）
- This polyclonal antibody is specific for human ZBP-89.
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产品特性
- Synonyms: rabbit anti-ZBP-89 Antibody, Transcription factor ZBP89 antibody, Zinc finger DNA binding protein 89 antibody, Zinc finger protein 148 antibody, ZNF 148 antibody, Zinc finger protein 148, Transcription factor ZBP-89, ZNF148
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纯化方法
- Anti-ZBP89 antibody was prepared from monospecific antiserum by delipidation and defibrination.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Purified full length ZBP-89 recombinant protein expressed in E.coli.
Immunogen Type: Recombinant Protein
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anti-Zinc Finger Protein 148 (ZNF148) antibody
ZNF148 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger Protein 148 (ZNF148) (AA 690-794) antibodyZNF148 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger Protein 148 (ZNF148) (N-Term) antibodyZNF148 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger Protein 148 (ZNF148) (N-Term) antibodyZNF148 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger Protein 148 (ZNF148) (AA 690-794) antibodyZNF148 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger Protein 148 (ZNF148) (AA 106-135), (N-Term) antibodyZNF148 适用: 人 WB 宿主: 兔 Polyclonal RB40518 unconjugated
anti-Zinc Finger Protein 148 (ZNF148) (N-Term) antibodyZNF148 适用: 人, Cow WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger Protein 148 (ZNF148) (N-Term) antibodyZNF148 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger Protein 148 (ZNF148) (AA 695-794) antibodyZNF148 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Zinc Finger Protein 148 (ZNF148) (AA 287-320) antibodyZNF148 适用: 斑马鱼 WB 宿主: 兔 Polyclonal RB46462 unconjugated
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应用备注
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Application Note: This polyclonal antibody reacts with human ZBP-89 in a variety of tested immunological assays including western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. For immunoblotting a 1:5,000 dilution is recommended. A band at approximately 89 kDa corresponding to human ZBP-89 is detected. Human monocytes or macrophages or nuclear extracts from PMA treated U937 cells can be used as a positive control. For ELISA a 1:10,000 to 1:30,000 dilution is recommended. Researchers should determine optimal titers for other applications.
Western Blot Dilution: 1:5,000
ELISA Dilution: 1:10,000 - 1:30,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 90 mg/mL
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缓冲液
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Buffer: None
Stabilizer: None
Preservative: 0.1 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store ZBP89 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Characterization of a functional ZBP-89 binding site that mediates Gata1 gene expression during hematopoietic development." in: The Journal of biological chemistry, Vol. 284, Issue 44, pp. 30187-99, (2009) (PubMed).
: "Characterization of a megakaryocyte-specific enhancer of the key hemopoietic transcription factor GATA1." in: The Journal of biological chemistry, Vol. 281, Issue 19, pp. 13733-42, (2006) (PubMed).
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: "Characterization of a functional ZBP-89 binding site that mediates Gata1 gene expression during hematopoietic development." in: The Journal of biological chemistry, Vol. 284, Issue 44, pp. 30187-99, (2009) (PubMed).
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- ZNF148 (Zinc Finger Protein 148 (ZNF148))
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别名
- ZNF148
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背景
- Background: The GI tract abundantly expresses growth factors many of which bind and activate the EGF receptor present on mucosal cells. One such factor is the zinc finger protein (ZBP-89) that binds to a GC-rich DNA element in the gastrin promoter and confers EGF responsiveness. The full-length protein functions as a repressor of growth factor signals regulating the gastrin promoter. Several other growth related promoters are also regulated by ZBP-89. ZBP-89 is one of a family of related transcriptional regulators. It has been reported in recent studies that ZBP-89 regulates growth in part by stimulating the cyclin-dependent kinase inhibitor, p21waf1, in a butyrate-dependent manner through recruitment of the histone acetyl transferase p300. Moreover, ZBP-89 triggers growth arrest in a p53-dependent manner by preventing nuclear export of p53. ZBP-89 also induces apoptosis, but this process occurs independent of p53.
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基因ID
- 256711
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UniProt
- Q9UQR1
抗原
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