GM-CSF 抗体
Quick Overview for GM-CSF 抗体 (ABIN964660)
抗原
See all GM-CSF (CSF2) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 209-401-314
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Supplier
- Rockland
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原理
- GM-CSF Antibody
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交叉反应 （详细）
- In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human GM-CSF in cell supernatants and certain body fluids.
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产品特性
- Synonyms: rabbit anti-GM-CSF Antibody, GM-CSF, rabbit anti-Colony-stimulating factor, CSF, sargramostim and molgramostin
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纯化方法
- This purified antibody has been heated to 56°C for 30 minutes.
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免疫原
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Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human GM-CSF protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 71-141) antibody
CSF2 适用: Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 65-144) antibodyCSF2 适用: 人 WB, ELISA, IF, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) antibodyCSF2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 59-85) antibodyCSF2 适用: 人 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal RB23360 unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) antibodyCSF2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 19-141) antibodyCSF2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) antibodyCSF2 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-141) antibodyCSF2 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 62-89), (Middle Region) antibodyCSF2 适用: 人 WB, FACS, IF, EIA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) antibodyCSF2 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This purified antibody has been tested for use in western blotting. Reactivity is also expected in neutralizations, ELISA, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/μl by the LAL method. By western blot a band approximately 14.5 kDa in size corresponding to human GM-CSF protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:1,000 - 1:5,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: None -
储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
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别名
- CSF2
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背景
- Background: Granulocyte Macrophage Colony Stimulating Factor (also known as GM-CSF, Colony-stimulating factor, CSF, sargramostim and molgramostin) is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors show significant homologies with other receptors for hematopoietic growth factors, including IL2-beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors.
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基因ID
- 1437
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NCBI登录号
- NP_000749
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UniProt
- P04141
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途径
- JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin
抗原
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