(2 references)-
- 抗原 See all ESRP2 抗体
- ESRP2 (Epithelial Splicing Regulatory Protein 2 (ESRP2))
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适用
- 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ESRP2 antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 特异性
- This product is an IgG fraction antibody purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above. This antibody reacts with mouse Esrp-2 protein. A BLAST analysis was used to suggest cross-reactivity with Esrp-2 from mouse based on a 100% homology with the immunizing sequence. Cross-reactivity with Esrp-2 from other sources has not been determined.
- 产品特性
- Epithelial splicing regulatory protein 2 (Esrp-2) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-2 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.
- 纯化方法
- purified
- 过滤
- Sterile filtered
- 免疫原
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Anti-Esrp-2 is produced by repeated immunizations of full length recombinant mouse Esrp-2 fusion protein.
Immunogen Type: RecombinantProtein - 克隆位点
- 22C5-H7-A6
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product ESRP2 Primary Antibody
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anti-Epithelial Splicing Regulatory Protein 2 (ESRP2) (AA 615-720) antibody
ESRP2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Splicing Regulatory Protein 2 (ESRP2) antibodyESRP2 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Splicing Regulatory Protein 2 (ESRP2) (AA 600-649) antibodyESRP2 适用: 人, 小鼠, 大鼠, 兔, Cow, 豚鼠, 猴, 马, Bat, Pig, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Splicing Regulatory Protein 2 (ESRP2) (AA 1-717) antibodyESRP2 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Epithelial Splicing Regulatory Protein 2 (ESRP2) (N-Term) antibodyESRP2 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Splicing Regulatory Protein 2 (ESRP2) (AA 61-110) antibodyESRP2 适用: 人, 小鼠, 大鼠, 兔, 犬, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Splicing Regulatory Protein 2 (ESRP2) (AA 36-85) antibodyESRP2 适用: 人, 小鼠, 大鼠, 兔, Cow, 豚鼠, 猴 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Anti-Esrp-2 protein A purified antibody has been tested for use western blotting and shows specific reactivity by with ESRP2 transfected cell lysates.. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 77.4 kDa in size corresponding to Esrp-2 by western blotting in the appropriate cell lysate or extract. The antibody shows bands at ~77kD and 70kD and no reactivity is observed in cells transfected with GFP or ESRP1. A starting dilution of 1:1000 is suggested for western blotting.
- 说明
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Gene Name: Esrp2
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
- 有效期
- 12 months
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: "Genome-wide screen for differentially methylated long noncoding RNAs identifies Esrp2 and lncRNA Esrp2-as regulated by enhancer DNA methylation with prognostic relevance for human breast cancer." in: Oncogene, Vol. 36, Issue 46, pp. 6446-6461, (2017) (PubMed).
: "An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition." in: The EMBO journal, Vol. 29, Issue 19, pp. 3286-300, (2010) (PubMed).
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: "Genome-wide screen for differentially methylated long noncoding RNAs identifies Esrp2 and lncRNA Esrp2-as regulated by enhancer DNA methylation with prognostic relevance for human breast cancer." in: Oncogene, Vol. 36, Issue 46, pp. 6446-6461, (2017) (PubMed).
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- 抗原
- ESRP2 (Epithelial Splicing Regulatory Protein 2 (ESRP2))
- 别名
- Esrp-2 (ESRP2 产品)
- 别名
- RBM35B antibody, 9530027K23Rik antibody, Rbm35b antibody, RGD1310855 antibody, cb404 antibody, fa07a06 antibody, rbm35b antibody, sb:cb404 antibody, zgc:77254 antibody, epithelial splicing regulatory protein 2 antibody, microRNA 6773 antibody, ESRP2 antibody, Esrp2 antibody, MIR6773 antibody, esrp2 antibody
- 背景
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Epithelial splicing regulatory protein 2 (Esrp-2) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-2 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.
Synonyms: RNA-binding protein 35B, RNA-binding motif protein 35B. - 基因ID
- 77411
- NCBI登录号
- NP_789808
- UniProt
- Q8K0G8
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