ESRP2 抗体
(2 references)
Quick Overview for ESRP2 抗体 (ABIN964643)
抗原
See all ESRP2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 210-301-c32
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Supplier
- Rockland
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原理
- Esrp-2 Antibody
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交叉反应 （详细）
- This antibody reacts with mouse Esrp-2 protein.
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产品特性
- Synonyms: mouse anti-Esrp2 antibody, mouse anti-Esrp-2 antibody, RNA-binding protein 35B, RNA-binding motif protein 35B
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纯化方法
- Anti-Esrp-2 is an IgG fraction antibody purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-Esrp-2 is produced by repeated immunizations of full length recombinant mouse Esrp-2 fusion protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG2a kappa
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anti-Epithelial Splicing Regulatory Protein 2 (ESRP2) (AA 615-720) antibody
ESRP2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Splicing Regulatory Protein 2 (ESRP2) antibodyESRP2 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Splicing Regulatory Protein 2 (ESRP2) (AA 600-649) antibodyESRP2 适用: 人, 小鼠, 大鼠, 兔, Cow, 豚鼠, 猴, 马, Bat, Pig, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Splicing Regulatory Protein 2 (ESRP2) (AA 1-717) antibodyESRP2 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Epithelial Splicing Regulatory Protein 2 (ESRP2) (N-Term) antibodyESRP2 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Splicing Regulatory Protein 2 (ESRP2) (AA 61-110) antibodyESRP2 适用: 人, 小鼠, 大鼠, 犬, 兔, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Splicing Regulatory Protein 2 (ESRP2) (AA 36-85) antibodyESRP2 适用: 人, 小鼠, 大鼠, 兔, Cow, 豚鼠, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Splicing Regulatory Protein 2 (ESRP2) (N-Term) antibodyESRP2 适用: 人, 小鼠, 大鼠, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Splicing Regulatory Protein 2 (ESRP2) (N-Term) antibodyESRP2 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Esrp-2 protein A purified antibody has been tested for use western blotting and ELISA. This antibody shows specific reactivity by with ESRP2 transfected cell lysates. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 77.4 kDa in size corresponding to Esrp-2 by western blotting in the appropriate cell lysate or extract. The antibody shows bands at ~77kD and 70kD and no reactivity is observed in cells transfected with GFP or ESRP1. A starting dilution of 1:1000 is suggested for western blotting.
Western Blot Dilution: 1:1000
ELISA Dilution: 1:100,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Genome-wide screen for differentially methylated long noncoding RNAs identifies Esrp2 and lncRNA Esrp2-as regulated by enhancer DNA methylation with prognostic relevance for human breast cancer." in: Oncogene, Vol. 36, Issue 46, pp. 6446-6461, (2017) (PubMed).
: "An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition." in: The EMBO journal, Vol. 29, Issue 19, pp. 3286-300, (2010) (PubMed).
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: "Genome-wide screen for differentially methylated long noncoding RNAs identifies Esrp2 and lncRNA Esrp2-as regulated by enhancer DNA methylation with prognostic relevance for human breast cancer." in: Oncogene, Vol. 36, Issue 46, pp. 6446-6461, (2017) (PubMed).
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- ESRP2 (Epithelial Splicing Regulatory Protein 2 (ESRP2))
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别名
- Esrp2
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背景
- Background: Epithelial splicing regulatory protein 2 (Esrp-2) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-2 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.
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基因ID
- 77411
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UniProt
- Q8K0G8
抗原
-
