ESRP1 抗体
(1 reference)
Our Local Distributor
北京 101111
Quick Overview for ESRP1 抗体 (ABIN964641)
抗原
See all ESRP1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
Supplier Product No.
- 210-301-c31
-
Supplier
- Rockland
-
原理
- Esrp-1/2 Antibody
-
交叉反应 （详细）
- This antibody reacts with both mouse Esrp-1 and Esrp-2 proteins.
-
产品特性
- Synonyms: mouse anti-Esrp1/2 antibody, mouse anti-Esrp1 antibody, mouse anti-Ersp2 antibody, Rbm35a, Epithelial splicing regulatory protein 1, RNA-binding protein 35A, RNA-binding motif protein 35A, RNA-binding protein 35B, RNA-binding motif protein 35B
-
纯化方法
- This antibody was purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above.
-
过滤
- Sterile filtered
-
免疫原
-
Immunogen: Anti-Esrp-1/2 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein.
Immunogen Type: Recombinant Protein
-
亚型
- IgG1
-
-
anti-Epithelial Splicing Regulatory Protein 1 (ESRP1) (AA 182-211) antibody
ESRP1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB27304 unconjugated
anti-Epithelial Splicing Regulatory Protein 1 (ESRP1) (AA 1-220) antibodyESRP1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Splicing Regulatory Protein 1 (ESRP1) (AA 1-681) antibodyESRP1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Epithelial Splicing Regulatory Protein 1 (ESRP1) (AA 161-210) antibodyESRP1 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 兔, Bat, 小鸡, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Splicing Regulatory Protein 1 (ESRP1) antibodyESRP1 适用: 小鼠 WB, ELISA 宿主: 小鼠 Monoclonal 27H12-F3-H5 unconjugated
anti-Epithelial Splicing Regulatory Protein 1 (ESRP1) (N-Term) antibodyESRP1 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF, EIA 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Application Note: This protein-A purified antibody has been tested for use western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 75.5 kDa in size corresponding to Esrp-1 and 77.4 kDa in size corresponding to Esrp-2 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1000
ELISA Dilution: 1:750,000
Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1.0 mg/mL
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
-
: "An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition." in: The EMBO journal, Vol. 29, Issue 19, pp. 3286-300, (2010) (PubMed).
-
: "An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition." in: The EMBO journal, Vol. 29, Issue 19, pp. 3286-300, (2010) (PubMed).
-
- ESRP1 (Epithelial Splicing Regulatory Protein 1 (ESRP1))
-
别名
- Esrp1/Esrp2
-
背景
- Background: Epithelial splicing regulatory protein-1 (Esrp-1) and Esrp-2 are mRNA splicing factors that regulate the formation of epithelial cell-specific isoforms. They specifically regulate the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-1 and -2 act by directly binding specific sequences in mRNAs. They bind the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.
-
基因ID
- 77411
-
NCBI登录号
- NP_789808
-
UniProt
- Q8K0G8
抗原
-
