ESRP1 抗体
(2 references)
Quick Overview for ESRP1 抗体 (ABIN964639)
抗原
See all ESRP1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 210-301-b89
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Supplier
- Rockland
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原理
- Esrp-1 Antibody
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交叉反应 （详细）
- This antibody reacts with mouse Esrp-1 protein.
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产品特性
- Synonyms: mouse anti-Esrp-1 antibody, mouse anti-Esrp1 antibody, Rbm35a, Epithelial splicing regulatory protein 1, RNA-binding protein 35A, RNA-binding motif protein 35A
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纯化方法
- This product is an IgG fraction antibody purified by Protein-A chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-Esrp-1 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG2b
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anti-Epithelial Splicing Regulatory Protein 1 (ESRP1) (AA 182-211) antibody
ESRP1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB27304 unconjugated
anti-Epithelial Splicing Regulatory Protein 1 (ESRP1) (AA 1-681) antibodyESRP1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Epithelial Splicing Regulatory Protein 1 (ESRP1) (AA 161-210) antibodyESRP1 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 兔, Bat, 小鸡, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Splicing Regulatory Protein 1 (ESRP1) (AA 1-220) antibodyESRP1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Splicing Regulatory Protein 1 (ESRP1) antibodyESRP1 适用: 小鼠 WB, ELISA 宿主: 小鼠 Monoclonal 23A7-C9 unconjugated
anti-Epithelial Splicing Regulatory Protein 1 (ESRP1) (N-Term) antibodyESRP1 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF, EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This protein-A purified antibody has been tested for use western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 75.5 kDa in size corresponding to Esrp-1 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1000
ELISA Dilution: 1:750,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Pressure-overload cardiac hypertrophy is associated with distinct alternative splicing due to altered expression of splicing factors." in: Molecules and cells, Vol. 37, Issue 1, pp. 81-7, (2014) (PubMed).
: "An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition." in: The EMBO journal, Vol. 29, Issue 19, pp. 3286-300, (2010) (PubMed).
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: "Pressure-overload cardiac hypertrophy is associated with distinct alternative splicing due to altered expression of splicing factors." in: Molecules and cells, Vol. 37, Issue 1, pp. 81-7, (2014) (PubMed).
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- ESRP1 (Epithelial Splicing Regulatory Protein 1 (ESRP1))
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别名
- Esrp1
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背景
- Background: Epithelial splicing regulatory protein 1 (Esrp-1) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). EsrpP-1 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.
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基因ID
- 207920
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NCBI登录号
- NP_918944
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UniProt
- Q3US41
抗原
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