Crasp-2 抗体
This anti-Crasp-2 antibody is a 兔 多克隆 antibody detecting Crasp-2 in ELISA 和 WB. Suitable for Borrelia burgdorferi.
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Quick Overview for Crasp-2 抗体 (ABIN964612)
抗原
Crasp-2
(Complement regulator-acquiring surface protein 2 (Crasp-2))
适用
Borrelia burgdorferi
宿主
兔
克隆类型
多克隆
应用范围
ELISA, Western Blotting (WB)
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Supplier Product No.
- 200-401-c19
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Supplier
- Rockland
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原理
- CRASP-2 Antibody
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交叉反应 （详细）
- This antibody is specific for Borrelia burgdorferi CRASP-2 protein.
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产品特性
- Synonyms: rabbit anti-CRASP-2 Antibody, Borrelia burgdorferi CRASP-2, CRASP2, CRASP 2
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纯化方法
- This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography.
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免疫原
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Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi CRASP-2 protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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应用备注
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Application Note: This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 25.4 kDa in size corresponding to Borrelia burgdorferi CRASP-2 protein by Western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1,000
ELISA Dilution: 1:2,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Crasp-2 (Complement regulator-acquiring surface protein 2 (Crasp-2))
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别名
- CRASP-2
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背景
- Background: CRASP-2 (Complement Regulator-Acquiring Surface Protein 2) of Borrelia burgdorferi binds FHL-1 and factor H binding protein in a distinct way. It may be predominantly expressed by serum-resistant Borrelia strains. Borrelia burgdorferi sensu lato has the ability to evade immune systems to persist in a variety of vertebrate hosts. This activity is dependent on a number of factors. Some Borrelia species bind host-derived fluid-phase immune regulators FHL-1 and factor H to their surface via complement regulator-acquiring surface proteins (CRASPs). Factor H and FHL-1 serve as cofactors for factor I, a serine protease that cleaves complement component 3b (C3b) directly on the cell surface and thereby confers resistance of spirochetes to complement-mediated lysis. It is possible that because of discontinuous binding regions in the factor H/FHL-1, long distance interaction may be involved in binding of both immune regulators. Putative coiled-coil structural elements may be important in the interaction of B. burgdorferi CRASP-1 with factor H.
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基因ID
- 1194149
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NCBI登录号
- WP_010890313
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UniProt
- O50665
抗原
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