hCAS5 (N-Term) 抗体
This anti- antibody is a 兔 多克隆 antibody detecting in ELISA 和 WB. Suitable for 人.
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Quick Overview for hCAS5 (N-Term) 抗体 (ABIN964597)
抗原
hCAS5
适用
人
宿主
兔
克隆类型
多克隆
应用范围
ELISA, Western Blotting (WB)
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抗原表位
- N-Term
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Supplier Product No.
- 600-401-948
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Supplier
- Rockland
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原理
- Cas5 Antibody
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交叉反应 （详细）
- This affinity-purified antibody is directed against human Cas5 protein.
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产品特性
- Synonyms: rabbit anti-Cas5 antibody, Castor homolog 1 zinc finger protein, CST, SRG, ZNF693, CASZ1, Cas-5, Cas 5
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to region near the amino terminal end of human Cas5 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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应用备注
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Application Note: This affinity-purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot, a band approximately 125 kDa in size corresponding to hCas5 protein is expected in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500- 1:2,000
ELISA Dilution: 1:10,000 - 1:50,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.3 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- hCAS5
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背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. hCas5 (Castor) is the human homolog of a Drosophila gene that regulates neurogenesis. In human neuro-blastoma tumors where a loss of heterozygosity of CASZ1 is noted, hCas functions to suppress the tumorigenicity of cells. hCas5 is a developmentally regulated transcription factor found in neural and muscle cells. hCas5 is a Zn finger transcription factor that is predominantly found in the nucleus. This antibody is designed to facilitate the functional study of this protein and its role in developmental regulation and expression in human tumor tissues.
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基因ID
- 54897
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NCBI登录号
- XP_016857029
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UniProt
- B3KRV8
抗原
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