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MYB 抗体 (pThr486)

Name: MYB 抗体 (pThr486)
SKU: ABIN964596
Availability: OutOfStock

MYB 适用: 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated

Western Blot of Rabbit c-Myb pT486 antibody. (MYB 抗体 (pThr486))

1 image

产品编号 ABIN964596

Supplier Product No.: 600-401-c47s

发货至: 中国

Datasheet as PDF

抗原 See all MYB 抗体

MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))
抗原表位
15

12

11

10

8

5

4

4

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pThr486
适用
112

53

9

8

7

5

5

5

4

3

2

2

2

1
小鼠
宿主
94

20

3

2
兔
Compatible Secondaries
克隆类型
98

21
多克隆
标记
81

5

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This MYB antibody is un-conjugated
应用范围
88

54

42

23

20

13

13

11

10

5

3

3

2

2

1

1
Western Blotting (WB), ELISA

Supplier Product No.

600-401-c47s

Supplier

Rockland

原理

C-Myb phosphoT486 Antibody

交叉反应（详细）

This affinity-purified antibody is directed against the phosphorylated form of mouse c-Myb protein at the pT486 residue.

产品特性

Synonyms: rabbit anti-c-Myb pT486 Antibody, Proto-oncogene c-Myb antibody, C myb antibody, MYB antibody, Transcriptional activator Myb, cmyb antibody

纯化方法

Antiserum was first purified against the phosphorylated form of the immunizing peptide.

过滤

Sterile filtered

免疫原

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse c-Myb protein surrounding amino acid residue 486.
Immunogen Type: Conjugated Peptide

亚型

IgG

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应用备注

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting using M1 cells which express endogenous wild-type c-Myb protein and in COS7 cells transfected with c-Myb. Specific conditions for reactivity and detection of phosphorylated c-Myb should be optimized by the end user. Expect a band approximately ~75 kDa in size corresponding to phosphorylated c-Myb by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:2,500
ELISA Dilution: 1:5,000-1:16,000
Other: User Optimized

限制

仅限研究用
状态

Liquid

浓度

0.91 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
抗原

MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))

别名

Myb (MYB 产品)

背景

Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The transcription factor c-Myb plays an important role in the development of all hematopoietic lineages during definitive hematopoiesis. Its critical role in hematopoiesis has been first demonstrated in experiments with targeted disruption of the c-Myb gene where deletion of both alleles of the c-Myb gene was embryonally lethal due to severe anemia. c-Myb is a short-lived sequence specific DNA-binding protein that regulates transcription of several important genes involved directly in cellular processes such as proliferation, differentiation, and apoptosis. Post-translational modifications such as phosphorylation, acetylation, ubiquitination, and conjugation of SUMO proteins are crucial for modulation of the transactivation activity of c-Myb. Several phosphorylation sites serve to regulate c-Myb activity.

基因ID

17863, 110556654

UniProt

P06876

途径

Chromatin Binding, Positive Regulation of Immune Effector Process