MYB 抗体 (pThr486)
Quick Overview for MYB 抗体 (pThr486) (ABIN964595)
抗原
See all MYB 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr486
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Supplier Product No.
- 600-401-c47
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Supplier
- Rockland
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原理
- C-Myb phosphoT486 Antibody
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交叉反应 （详细）
- This affinity-purified antibody is directed against the phosphorylated form of mouse c-Myb protein at the pT486 residue.
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产品特性
- Synonyms: rabbit anti-c-Myb pT486 Antibody, Proto-oncogene c-Myb antibody, C myb antibody, MYB antibody, Transcriptional activator Myb, cmyb antibody
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纯化方法
- Antiserum was first purified against the phosphorylated form of the immunizing peptide.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse c-Myb protein surrounding amino acid residue 486.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB) (AA 281-294) antibody
Verified MYB 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB) (C-Term) antibodyMYB 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB) (AA 1-640) antibodyMYB 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB) (C-Term) antibodyMYB 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB) (pSer532) antibodyMYB 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB) (N-Term) antibodyMYB 适用: 人, 小鼠 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB) (N-Term) antibodyMYB 适用: 人, 小鼠, 大鼠, 犬, Cow, 非洲爪蟾, 小鸡 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB) (AA 305-558) antibodyMYB 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB) (N-Term) antibodyMYB 适用: 人, 小鼠, 大鼠, Cow WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB) (AA 1-50) antibodyMYB 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting using M1 cells which express endogenous wild-type c-Myb protein and in COS7 cells transfected with c-Myb. Specific conditions for reactivity and detection of phosphorylated c-Myb should be optimized by the end user. Expect a band approximately ~75 kDa in size corresponding to phosphorylated c-Myb by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:2,500
ELISA Dilution: 1:5,000-1:16,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.91 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- MYB (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB))
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别名
- Myb
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背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The transcription factor c-Myb plays an important role in the development of all hematopoietic lineages during definitive hematopoiesis. Its critical role in hematopoiesis has been first demonstrated in experiments with targeted disruption of the c-Myb gene where deletion of both alleles of the c-Myb gene was embryonally lethal due to severe anemia. c-Myb is a short-lived sequence specific DNA-binding protein that regulates transcription of several important genes involved directly in cellular processes such as proliferation, differentiation, and apoptosis. Post-translational modifications such as phosphorylation, acetylation, ubiquitination, and conjugation of SUMO proteins are crucial for modulation of the transactivation activity of c-Myb. Several phosphorylation sites serve to regulate c-Myb activity.
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基因ID
- 17863, 110556654
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UniProt
- P06876
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途径
- Chromatin Binding, Positive Regulation of Immune Effector Process
抗原
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