TUBA1B 抗体 (C-Term)
(6 references)
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Quick Overview for TUBA1B 抗体 (C-Term) (ABIN964563)
抗原
See all TUBA1B 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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Supplier Product No.
- 200-301-880
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Supplier
- Rockland
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原理
- Alpha-Tubulin Antibody
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交叉反应 （详细）
- This Loading Control antibody is directed against alpha tubulin.
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产品特性
- Synonyms: mouse anti-Alpha-Tubulin Antibody, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, TUBA1B, tubulin loading control
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纯化方法
- Anti-Tubulin Loading Control Antibody was purified by Protein A chromatography.
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过滤
- Sterile filtered
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免疫原
- Optional[Immunogen]: Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human alpha tubulin protein.
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亚型
- IgG1 kappa
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anti-Tubulin, alpha 1B (TUBA1B) antibody
TUBA1B 适用: 人 WB, IF, IHC (p), ICC, IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 1-451) antibodyTUBA1B 适用: 人 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 1-451) antibodyTUBA1B 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4D1 unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 425-451) antibodyTUBA1B 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC, FM 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 29-310) antibodyTUBA1B 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 1-451) antibodyTUBA1B 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2E8 unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (C-Term) antibodyTUBA1B 适用: 人, 小鼠, 大鼠, 小鸡, Cow WB, ELISA, IF, IHC, IHC (p) 宿主: 小鼠 Monoclonal 17H11-F10 unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 1-451) antibodyTUBA1B 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) antibodyTUBA1B 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 1-451) antibodyTUBA1B 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 2.5 μg/mL
Application Note: Anti-Tubulin Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.
Western Blot Dilution: 1:1,000
ELISA Dilution: 1:300,000
IF Microscopy Dilution: 0.1 μg/mL
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store Anti-Tubulin Antibody at -20° C prior to opening. Aliquot Loading Control Antibody and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge Tubulin Loading Control Antibody if not completely clear after standing at room temperature. This Anti-Tubulin Loading Control Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Canonical and noncanonical TGF-β signaling regulate fibrous tissue differentiation in the axial skeleton." in: Scientific reports, Vol. 10, Issue 1, pp. 21364, (2021) (PubMed).
: "SOX9 protein is stabilized by TGF-β and regulates PAPSS2 mRNA expression in chondrocytes." in: Osteoarthritis and cartilage, Vol. 25, Issue 2, pp. 332-340, (2018) (PubMed).
: "The Common R71H-G230A-R293Q Human TMEM173 Is a Null Allele." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 198, Issue 2, pp. 776-787, (2017) (PubMed).
: "Palmitoylation of the ciliary GTPase ARL13b is necessary for its stability and its role in cilia formation." in: The Journal of biological chemistry, Vol. 292, Issue 43, pp. 17703-17717, (2017) (PubMed).
: "CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes." in: Nature structural & molecular biology, Vol. 22, Issue 5, pp. 396-403, (2015) (PubMed).
: "MPYS/STING-mediated TNF-α, not type I IFN, is essential for the mucosal adjuvant activity of (3'-5')-cyclic-di-guanosine-monophosphate in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 1, pp. 492-502, (2014) (PubMed).
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: "Canonical and noncanonical TGF-β signaling regulate fibrous tissue differentiation in the axial skeleton." in: Scientific reports, Vol. 10, Issue 1, pp. 21364, (2021) (PubMed).
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- TUBA1B (Tubulin, alpha 1B (TUBA1B))
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别名
- TUBA1B
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背景
- Background: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control.
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基因ID
- 17986283
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UniProt
- P68363
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途径
- Microtubule Dynamics, M Phase
抗原
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