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TUBA1B 抗体 (C-Term)

Name: TUBA1B 抗体 (C-Term)
SKU: ABIN964563
Availability: OutOfStock

This anti-TUBA1B antibody is a 小鼠单克隆 antibody detecting TUBA1B in WB, ELISA, IHC, IF, FM 和 MA. Suitable for 人. This Primary Antibody has been cited in 6+ publications.

Scx is required for the expression of Fmod and Adamtsl2. (TUBA1B 抗体 (C-Term))

Immunofluorescence (IF) image for anti-Tubulin, alpha 1B (TUBA1B) (C-Term) antibody (ABIN964563) (TUBA1B 抗体 (C-Term))

Immunofluorescence of Mouse monoclonal anti-Alpha Tubulin antibody. (TUBA1B 抗体 (C-Term))

Immunofluorescences of Mouse monoclonal anti-Alpha Tubulin antibody. (TUBA1B 抗体 (C-Term))

HeLa whole cell lysate (left lane) and HEK293 whole cell lysate (right lane) were loaded with 10 µg of lysate each. (TUBA1B 抗体 (C-Term))

Smad3 is required for TGF-β to regulate expression of Fmod and Adamtsl2. (TUBA1B 抗体 (C-Term))

TGF-β signaling regulates noncanonical pathways in the sclerotome. (TUBA1B 抗体 (C-Term))

AKT and p38 are not required for TGF-β-mediated regulation of fibrous tissue markers. (TUBA1B 抗体 (C-Term))

ERK is required for fibrous tissue marker regulation but not required to inhibit chondrogenesis. (TUBA1B 抗体 (C-Term))

13 images

(6 references)

产品编号 ABIN964563

Supplier Product No.: 200-301-880

发货至: 中国

Datasheet as PDF

Quick Overview for TUBA1B 抗体 (C-Term) (ABIN964563)

抗原

See all TUBA1B 抗体

TUBA1B (Tubulin, alpha 1B (TUBA1B))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This TUBA1B antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM), Multiplex Assay (MA)

克隆位点

17H11-F10

Discover our top product TUBA1B Primary Antibody

抗原表位
15

7

5

3

3

3

1

1

1

1

1
C-Term

Supplier Product No.

200-301-880

Supplier

Rockland

原理

Alpha-Tubulin Antibody

交叉反应（详细）

This Loading Control antibody is directed against alpha tubulin.

产品特性

Synonyms: mouse anti-Alpha-Tubulin Antibody, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, TUBA1B, tubulin loading control

纯化方法

Anti-Tubulin Loading Control Antibody was purified by Protein A chromatography.

过滤

Sterile filtered

免疫原

Optional[Immunogen]: Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human alpha tubulin protein.

亚型

IgG1 kappa
anti-Tubulin, alpha 1B (TUBA1B) antibody
TUBA1B 适用: 人 WB, IF, IHC (p), ICC, IHC (wm) 宿主: 兔 Polyclonal unconjugated

anti-Tubulin, alpha 1B (TUBA1B) (AA 1-451) antibody
TUBA1B 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4D1 unconjugated

anti-Tubulin, alpha 1B (TUBA1B) (AA 425-451) antibody
TUBA1B 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, FM 宿主: 兔 Polyclonal unconjugated

anti-Tubulin, alpha 1B (TUBA1B) (C-Term) antibody
TUBA1B 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Tubulin, alpha 1B (TUBA1B) (AA 29-310) antibody
TUBA1B 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Tubulin, alpha 1B (TUBA1B) (AA 1-451) antibody
TUBA1B 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2E8 unconjugated

anti-Tubulin, alpha 1B (TUBA1B) (C-Term) antibody
TUBA1B 适用: 人, 小鼠, 大鼠, 小鸡, Cow WB, ELISA, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 17H11-F10 unconjugated

anti-Tubulin, alpha 1B (TUBA1B) (AA 437-441) antibody
TUBA1B 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Tubulin, alpha 1B (TUBA1B) (AA 1-451) antibody
TUBA1B 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-Tubulin, alpha 1B (TUBA1B) (N-Term) antibody
TUBA1B 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated

应用备注

Immunohistochemistry Dilution: 2.5 μg/mL
Application Note: Anti-Tubulin Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.
Western Blot Dilution: 1:1,000
ELISA Dilution: 1:300,000
IF Microscopy Dilution: 0.1 μg/mL
Other: User Optimized

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store Anti-Tubulin Antibody at -20° C prior to opening. Aliquot Loading Control Antibody and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge Tubulin Loading Control Antibody if not completely clear after standing at room temperature. This Anti-Tubulin Loading Control Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Clayton, Ban, Liu, Serra: "Canonical and noncanonical TGF-β signaling regulate fibrous tissue differentiation in the axial skeleton." in: Scientific reports, Vol. 10, Issue 1, pp. 21364, (2021) (PubMed).

Chavez, Coricor, Perez, Seo, Serra: "SOX9 protein is stabilized by TGF-β and regulates PAPSS2 mRNA expression in chondrocytes." in: Osteoarthritis and cartilage, Vol. 25, Issue 2, pp. 332-340, (2018) (PubMed).

Patel, Blaauboer, Tucker, Mansouri, Ruiz-Moreno, Hamann, Schumann, Opitz, Jin: "The Common R71H-G230A-R293Q Human TMEM173 Is a Null Allele." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 198, Issue 2, pp. 776-787, (2017) (PubMed).

Roy, Jerman, Jozsef, McNamara, Onyekaba, Sun, Marin: "Palmitoylation of the ciliary GTPase ARL13b is necessary for its stability and its role in cilia formation." in: The Journal of biological chemistry, Vol. 292, Issue 43, pp. 17703-17717, (2017) (PubMed).

Laitem, Zaborowska, Isa, Kufs, Dienstbier, Murphy: "CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes." in: Nature structural & molecular biology, Vol. 22, Issue 5, pp. 396-403, (2015) (PubMed).

Blaauboer, Gabrielle, Jin: "MPYS/STING-mediated TNF-α, not type I IFN, is essential for the mucosal adjuvant activity of (3'-5')-cyclic-di-guanosine-monophosphate in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 1, pp. 492-502, (2014) (PubMed).
抗原

TUBA1B (Tubulin, alpha 1B (TUBA1B))

别名

TUBA1B

背景

Background: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control.

基因ID

17986283

UniProt

P68363

途径

Microtubule Dynamics, M Phase