AHSA1 抗体
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- 抗原 See all AHSA1 抗体
- AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))
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适用
- 人, 小鼠, Chimpanzee
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This AHSA1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 特异性
- This product is an IgG fraction antibody purified from tissue culture supernatant by Protein-G chromatography, followed by extensive dialysis against the buffer stated above. It is directed against human AHA1 protein. A BLAST analysis was used to suggest cross-reactivity with AHA1 protein from mouse, human and chimpanzee based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
- 交叉反应
- 人, Chimpanzee
- 产品特性
- Activator of Hsp90 ATPase (AHA1) stimulates the inherent ATPase cycle of Hsp90, which is essential for its chaperone activity in vivo. The activation and/or stability of many of the key regulatory and signaling proteins of the eukaryotic cell depend on their interaction with the Hsp90 molecular chaperone. Hsp90 is assisted and regulated by co-chaperones that participate in an ordered series both to assist client-protein recruitment or release and to modulate progress through the ATPase coupled chaperone cycle. Structural analysis and mutagenesis show that binding of the N-terminal domain of AHA1 to Hsp90 promotes a conformational switch in the middle-segment catalytic loop (aa 370-390) of Hsp90 that exposes the catalytic Arg380 and enables its interaction with ATP in the N-terminal nucleotide-binding domain of the chaperone. Recent studies show that AHA1 modulates Hsp90-dependent stability of the folding of the cystic fibrosis transmembrane conductance regulator (CFTR) in the endoplasmic reticulum (ER). Down-regulation of AHA1 rescues misfolding of CFTR in cystic fibrosis.
- 纯化方法
- affinity purified
- 过滤
- Sterile filtered
- 免疫原
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This Protein G purified monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse AHA1 protein followed by hybridoma development.
Immunogen Type: RecombinantProtein - 克隆位点
- 25F2-D9
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product AHSA1 Primary Antibody
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anti-Activator of HSP90 ATPase Activity 1 (AHSA1) (Internal Region) antibody
AHSA1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) (AA 1-338) antibodyAHSA1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A2-A8 unconjugated
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) (AA 1-338) antibodyAHSA1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 4F3-B6 unconjugated
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) antibodyAHSA1 适用: 小鼠 WB, ELISA, IHC, IP, IF, ICC 宿主: 大鼠 Monoclonal 25F2-D9 unconjugated
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) (Center) antibodyAHSA1 适用: 人, 小鼠 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) antibody (HRP)AHSA1 适用: 小鼠 WB, ELISA, IHC, IP, IF, ICC 宿主: 大鼠 Monoclonal 25F2-D9 HRP
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) antibody (FITC)AHSA1 适用: 小鼠 WB, ELISA, IHC, IP, IF, ICC 宿主: 大鼠 Monoclonal 25F2-D9 FITC
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) antibody (Biotin)AHSA1 适用: 小鼠 WB, ELISA, IHC, IP, IF, ICC 宿主: 大鼠 Monoclonal 25F2-D9 Biotin
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) antibody (PE)AHSA1 适用: 小鼠 WB, ELISA, IHC, IP, IF, ICC 宿主: 大鼠 Monoclonal 25F2-D9 PE
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) antibody (APC)AHSA1 适用: 小鼠 WB, ELISA, IHC, IP, IF, ICC 宿主: 大鼠 Monoclonal 25F2-D9 APC
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- 应用备注
- This Protein-G purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38-40 kDa in size corresponding to AHA1 protein by western blotting in the appropriate cell lysate or extract.
- 说明
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Gene Name: Ahsa1
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
- 有效期
- 12 months
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- 抗原
- AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))
- 别名
- AHA1 (AHSA1 产品)
- 别名
- ahsa1 antibody, MGC52884 antibody, MGC132147 antibody, MGC80312 antibody, AHSA1 antibody, MGC89589 antibody, wu:fb83b05 antibody, zgc:136821 antibody, zgc:56075 antibody, AHA1 antibody, C14orf3 antibody, p38 antibody, BC023857 antibody, AHA1, activator of heat shock 90kDa protein ATPase homolog 1 S homeolog antibody, activator of Hsp90 ATPase activity 1 antibody, AHA1, activator of heat shock 90kDa protein ATPase homolog 1 L homeolog antibody, activator of HSP90 ATPase activity 1 antibody, AHA1, activator of heat shock 90kDa protein ATPase homolog 1 antibody, AHA1, activator of heat shock protein ATPase homolog 1a antibody, AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast) antibody, AHA1, activator of heat shock protein ATPase 1 antibody, ahsa1.S antibody, AHSA1 antibody, ahsa1.L antibody, ahsa1 antibody, ahsa1a antibody, Ahsa1 antibody
- 背景
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Activator of Hsp90 ATPase (AHA1) stimulates the inherent ATPase cycle of Hsp90, which is essential for its chaperone activity in vivo. The activation and/or stability of many of the key regulatory and signaling proteins of the eukaryotic cell depend on their interaction with the Hsp90 molecular chaperone. Hsp90 is assisted and regulated by co-chaperones that participate in an ordered series both to assist client-protein recruitment or release and to modulate progress through the ATPase coupled chaperone cycle. Structural analysis and mutagenesis show that binding of the N-terminal domain of AHA1 to Hsp90 promotes a conformational switch in the middle-segment catalytic loop (aa 370–390) of Hsp90 that exposes the catalytic Arg380 and enables its interaction with ATP in the N-terminal nucleotide-binding domain of the chaperone. Recent studies show that AHA1 modulates Hsp90-dependent stability of the folding of the cystic fibrosis transmembrane conductance regulator (CFTR) in the endoplasmic reticulum (ER). Down-regulation of AHA1 rescues misfolding of CFTR in cystic fibrosis.
Synonyms: Aha1, Ahsa1 antibody, Activator of Hsp90 ATPase, Activator of 90 kDa heat shock protein ATPase homolog 1 antibody - 基因ID
- 217737
- NCBI登录号
- NP_666148
- UniProt
- Q8BK64
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