AHSA1 抗体
Quick Overview for AHSA1 抗体 (ABIN964557)
抗原
See all AHSA1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-501-974
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Supplier
- Rockland
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原理
- Aha1 Antibody
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交叉反应 （详细）
- It is directed against human Aha1 protein.
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产品特性
- Synonyms: rat anti-Aha1 Antibody, Aha1, Ahsa1 antibody, Activator of Hsp90 ATPase, Activator of 90 kDa heat shock protein ATPase homolog 1 antibody
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纯化方法
- This product is an IgG fraction antibody purified from tissue culture supernatant by Protein-G chromatography, followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This Protein G purified monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse AHA1 protein followed by hybridoma development.
Immunogen Type: Recombinant Protein
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亚型
- IgG2a kappa
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anti-Activator of HSP90 ATPase Activity 1 (AHSA1) (Internal Region) antibody
AHSA1 适用: 人, 猴 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) (AA 1-338) antibodyAHSA1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A2-A8 unconjugated
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) (AA 1-338) antibodyAHSA1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 4F3-B6 unconjugated
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) (Center) antibodyAHSA1 适用: 人, 小鼠 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) antibodyAHSA1 适用: 小鼠 WB, ELISA, IHC, IP, IF, ICC 宿主: 大鼠 Monoclonal 25F2-D9 unconjugated
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) antibody (HRP)AHSA1 适用: 小鼠 WB, ELISA, IHC, IP, IF, ICC 宿主: 大鼠 Monoclonal 25F2-D9 HRP
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) antibody (FITC)AHSA1 适用: 小鼠 WB, ELISA, IHC, IP, IF, ICC 宿主: 大鼠 Monoclonal 25F2-D9 FITC
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) antibody (Biotin)AHSA1 适用: 小鼠 WB, ELISA, IHC, IP, IF, ICC 宿主: 大鼠 Monoclonal 25F2-D9 Biotin
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) antibody (PE)AHSA1 适用: 小鼠 WB, ELISA, IHC, IP, IF, ICC 宿主: 大鼠 Monoclonal 25F2-D9 PE
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) antibody (APC)AHSA1 适用: 小鼠 WB, ELISA, IHC, IP, IF, ICC 宿主: 大鼠 Monoclonal 25F2-D9 APC
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应用备注
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Immunohistochemistry Dilution: 5-10 μg/mL
Application Note: This Protein-G purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38-40 kDa in size corresponding to Aha1 protein by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1,000
ELISA Dilution: 1:20,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: None -
储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))
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别名
- Ahsa1
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背景
- Background: Activator of Hsp90 ATPase (AHA1) stimulates the inherent ATPase cycle of Hsp90, which is essential for its chaperone activity in vivo. The activation and/or stability of many of the key regulatory and signaling proteins of the eukaryotic cell depend on their interaction with the Hsp90 Molecular chaperone. Hsp90 is assisted and regulated by co-chaperones that participate in an ordered series both to assist client-protein recruitment or release and to modulate progress through the ATPase coupled chaperone cycle. Structural analysis and mutagenesis show that binding of the N-terminal domain of AHA1 to Hsp90 promotes a conformational switch in the middle-segment catalytic loop (aa 370-390) of Hsp90 that exposes the catalytic Arg380 and enables its interaction with ATP in the N-terminal nucleotide-binding domain of the chaperone. Recent studies show that AHA1 modulates Hsp90-dependent stability of the folding of the cystic fibrosis transmembrane conductance regulator (CFTR) in the endoplasmic reticulum (ER). Down-regulation of AHA1 rescues misfolding of CFTR in cystic fibrosis.
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基因ID
- 217737
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NCBI登录号
- NP_666148
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UniProt
- Q8BK64
抗原
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